DISTINCTIVE FUNCTIONAL-CHARACTERISTICS OF HUMAN LYMPHOCYTES-T DEFINED BY E-ROSETTING OR A MONOCLONAL ANTI-T-CELL ANTIBODY
DISTINCTIVE FUNCTIONAL-CHARACTERISTICS OF HUMAN LYMPHOCYTES-T DEFINED BY E-ROSETTING OR A MONOCLONAL ANTI-T-CELL ANTIBODY
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DOI：
10.1002/eji.1830110412
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发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
CALLARD, RE
中科院分区：
文献类型：
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作者：
BEVERLEY, PCL;CALLARD, RE
比较了羊红细胞（E）玫瑰花环法和UCHT 1单克隆抗T细胞抗体染色后荧光激活细胞分选法分离的人淋巴细胞组分的性质。鉴定了具有非常不同的表型和功能的两个E+细胞群体。E+/UCHT 1+细胞对T细胞有丝分裂原植物血凝素和伴刀豆球蛋白A反应良好，并为体外特异性抗体反应提供帮助。它们还可以抑制同种异体外周血单个核细胞的抗体应答。E+/UCHT 1-群体没有T细胞的其他标志物特征，不能对有丝分裂原作出反应，也不能为抗体反应提供帮助或抑制。这些细胞是非常活跃的自然杀手。它们具有Fc γ。受体，并具有非特异性酯酶活性的特征性染色模式。显然，并非所有能够形成雌激素的细胞都是胸腺加工细胞。这种异质性可以通过用单克隆抗T细胞试剂UCHT 1染色来揭示。
The properties of humam lymphocyte fractions isolated either by sheep red cell (E) rosetting or by fluorescence-activated cell sorting after staining with UCHT1 monoclonal anti-T cell antibody were compared. Two populations of E+ cells with very different phenotype and function were identified. E+/UCHT1+ cells respond well to the T cell mitogens phytohemagglutinin and concanavalin A and provide help for an in vitro specific antibody response. They can also suppress the antibody response of allogeneic peripheral blood mononuclear cells. The E+/UCHT1- population, which has no other markers characteristic of T cells, fails to respond to mitogens or to provide help or suppression for an antibody response. These cells are highly active natural killers. They possess Fc.gamma. receptors and have a characteristic staining pattern of nonspecific esterase enzyme activity. Evidently not all cells capable of forming E rosettes are thymus-processed cells. This heterogeneity can be revealed by staining with the monoclonal anti-T cell reagent UCHT1.