Differential expression of leukocyte-associated Ig-like receptor-1 during neutrophil differentiation and activation
Differential expression of leukocyte-associated Ig-like receptor-1 during neutrophil differentiation and activation
复制标题
DOI：
10.1189/jlb.0705370
复制
发表时间：
2006-04-01
影响因子：
5.5
通讯作者：
Meyaard, Linde
中科院分区：
文献类型：
--
作者：
Verbrugge, Annelies;de Ruiter, Talitha;Meyaard, Linde
含有免疫受体酪氨酸基序的抑制性受体在免疫细胞活化中起重要的调节作用。此外，一些研究表明，这些受体参与造血细胞分化的调节。在此，我们研究了白细胞相关免疫球蛋白样受体-1（LAIR-1）在中性粒细胞分化和活化中的表达，LAIR-1是一种在大多数外周血白细胞和CD 34（+）造血祖细胞上表达的抑制性受体。我们发现，虽然LAIR-1表达于外周血嗜酸性粒细胞，但成熟中性粒细胞的细胞表面表达较低，表明LAIR-1的表达在粒细胞分化过程中受到调节。事实上，早幼粒细胞系HL-60表达LAIR-1，但在向中性粒细胞的化学诱导分化过程中表达降低。类似地，在骨髓来源的中性粒细胞前体中，最不成熟的细胞表达LAIR-1，LAIR-1表达的丧失与中性粒细胞成熟相关。在用肿瘤坏死因子α、粒细胞巨噬细胞集落刺激因子或N-甲酰甲硫氨酰-亮氨酰-苯丙氨酸刺激后，LAIR-1在成熟中性粒细胞的膜上迅速重新表达，表明LAIR-1也可调节中性粒细胞效应子功能。我们的研究表明，LAIR-1可能在人粒细胞的分化和功能中起调节作用。
Inhibitory receptors containing immunoreceptor tyrosine-based inhibitory motifs play an important regulatory role in immune cell activation. In addition, several studies suggest that these receptors are involved in the regulation of hematopoietic cell differentiation. Here, we have investigated the expression of leukocyte-associated immunoglobulin-like receptor-1 (LAIR-1), an inhibitory receptor expressed on most peripheral blood leukocytes and on CD34(+) hematopoietic progenitor cells, in neutrophil differentiation and activation. We found that although LAIR-1 was expressed on peripheral blood eosinophils, cellsurface expression on mature neutrophils was low, suggesting that LAIR-1 expression is regulated during granulocyte differentiation. Indeed, the promyeloid cell line HL-60 expressed LAIR-1, but the expression decreased during chemical-induced differentiation toward neutrophils. Similarly, in bone marrow-derived neutrophil precursors, the most immature cells expressed LAIR-1, and loss of LAIR-1 expression was associated with neutrophil maturation. LAIR-1 was re-expressed rapidly on the membrane of mature neutrophils upon stimulation with tumor necrosis factor alpha, granulocyte macrophage-colony stimulating factor, or N-formylmethionyl-leucyl-phenylaianine, indicating that LAIR-1 may also regulate neutrophil effector function. Our studies suggest that LAIR-1 may play a regulatory role in differentiation and function of human granulocytes.