RELIABLE PHOTOMICROGRAPHIC METHOD FOR DETERMINING FAT-CELL SIZE AND NUMBER - APPLICATION TO DIETARY OBESITY
RELIABLE PHOTOMICROGRAPHIC METHOD FOR DETERMINING FAT-CELL SIZE AND NUMBER - APPLICATION TO DIETARY OBESITY
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DOI：
10.3181/00379727-156-39916
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发表时间：
1977-01-01
影响因子：
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通讯作者：
GREENWOOD, MRC
中科院分区：
文献类型：
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作者：
LAVAU, M;SUSINI, C;GREENWOOD, MRC
大鼠和人的分离脂肪细胞的大小是通过在光学显微镜上测量它们的直径和通过对Os固定的细胞进行电子计数来确定的。平均细胞大小分别为0.348微克和0.353微克脂肪/细胞。两种方法之间的一致性在广泛的细胞大小范围内(0.15-0.09微克脂肪/细胞)是很好的。应用显微照相技术，对饲喂高脂饲料1个月和10个月的Wistar大鼠附睾部脂肪组织进行了形态观察。1个月后脂肪库增大。高脂肪饮食仅通过增加脂肪细胞的大小来实现。10个月的高脂饮食导致了中度肥胖和重度肥胖，前者仅表现为脂肪细胞肥大，后者表现为明显的肥大-增生性改变。
Rat and human isolated adipocyte sizes were determined by the measurement of their diameter on photomicrographs and by electronic counting of Os-fixed cells. The average cell size was 0.348 and 0.353 .mu.g of lipid/cell, respectively. The agreement between the 2 methods was excellent over a wide range of cell sizes (0.15-0.09 .mu.g of lipid/cell). The photomicrographic method was applied to a morphological study of epididymal adipose tissue in Wistar rats fed a high fat diet for either 1 or 10 mo. The enlargement of the adipose depot induced by 1 mo. of the high fat diet was achieved by an increase of fat cell size only. Ten months of high-fat feeding induced either a moderate obesity with only hypertrophy of the adipocytes or a severe obesity with apparent hypertrophic-hyperplastic changes.