Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance)
Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (type II resistance)
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DOI：
10.1007/s001220200009
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发表时间：
2002-01-01
影响因子：
5.4
通讯作者：
Ruckenbauer, P
中科院分区：
文献类型：
--
作者：
Buerstmayr, H;Lemmens, M;Ruckenbauer, P
赤霉病是一种侵染小麦和其他小杂粮的真菌病害，广泛分布于温带和亚热带地区。赤霉病造成严重的产量和质量损失，但最严重的问题是谷物食品和饲料可能受到霉菌毒素污染。用常规选择方法选育赤霉病抗性是可行的，但繁琐而昂贵。本研究旨在寻找与小麦赤霉病抗性基因相关的DNA标记。在田间条件下，对来自‘CM-82036’(抗病)/‘Remus’(感病)的364个F-1衍生的双单倍体(DH)群体进行了2年的II型抗性鉴定。对随机选择的239个双亲进行了标记分析。应用了不同的标记类型，重点是AFLP和SSR标记。采用方差分析、简单区间作图和复合区间作图。有三个基因组区域与赤霉病抗性显著相关。在3B染色体的短臂上检测到最显著的效应，解释了高达60%的II型赤霉病抗性的表型变异。另外一个QTL位于5A染色体上，第三个QTL位于1B染色体上。利用SSR标记对3B和5A上的QTL区域进行标记，发现1B的QTL与高分子量谷蛋白基因座连锁。这些结果表明，赤霉病抗性是由几个主效QTL和数量未知的微效基因共同控制的。利用分子标记辅助选择这些与赤霉病抗性有关的主效QTL似乎是可行的，可以加速小麦品种的选育和农艺改良。
Fusarium head blight (FHB, scab) is a fungal disease of wheat and other small cereals that is found in both temperate and semi-tropical regions. FHB causes severe yield and quality losses, but the most-serious concern is the possible mycotoxin contamination of cereal food and feed. Breeding for FHB resistance by conventional selection is feasible, but tedious and expensive. This study was conducted to identify and map DNA markers associated with FHB resistance genes in wheat. A population of 364 F-1-derived doubled-haploid (DH) lines from the cross 'CM-82036' (resistant)/'Remus' (susceptible) was evaluated for Type II resistance (spread within the spike) during 2 years under field conditions. Marker analysis was performed on 239 randomly chosen DH lines. Different marker types were applied, with an emphasis on AFLP and SSR markers. Analysis of variance, as well as simple and composite interval mapping, were applied. Three genomic regions were found significantly associated with FHB resistance. The most-prominent effect was detected on the short arm of chromosome 3B, explaining up to 60% of the phenotypic variance for Type II FHB resistance. A further QTL was located on chromosome 5A and a third one on 1B. The QTL regions on 3B and 5A were tagged with flanking SSR markers, the 1B QTL was found associated with the high-molecular-weight glutenin locus. These results indicate that FHB resistance is under control of a few major QTLs operating together with unknown numbers of minor genes. Marker-assisted selection for these major QTLs involved in FHB resistance appears feasible and should accelerate the development or resistant and agronomically improved wheat cultivars.