Three-dimensional measurement of ice crystals in frozen dilute solution
Three-dimensional measurement of ice crystals in frozen dilute solution
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DOI：
10.1016/s0140-7007(03)00043-4
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发表时间：
2004-05
期刊：
影响因子：
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通讯作者：
S. Ueno;G. Do;Y. Sagara;K. Kudoh;T. Higuchi
中科院分区：
文献类型：
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作者：
S. Ueno;G. Do;Y. Sagara;K. Kudoh;T. Higuchi
利用显微切片图像处理系统（MSIPS）对冷冻稀溶液中的冰晶结构进行了观测。基于对厚度为5 μm的冰冻样品进行多切片后得到的冰晶三维重建图像，提出了几个表征冰晶三维形态和分布的特征参数。对尺寸为530×700×1000 μm的样品图像进行了特征参数的测定。在任何冻结条件下，冰晶的三维形态都是一束连续的或树枝状的柱状体。冰晶的当量直径在73-169 μm之间，在铜冷却板温度为-20 ° C至-80 °C时，冰晶的当量直径随冻结速率的增加呈指数下降。在Tcp−40 °C时，冰晶的体积在4.6×104μ m3到3.3×107μm3之间，在三维图像中计数到36个冰柱。
A Micro-Slicer Image Processing System (MSIPS) has been applied to observe the ice crystal structures formed in frozen dilute solutions. Several characteristic parameters were also proposed to investigate the three-dimensional (3-D) morphology and distribution of ice crystals, based on their reconstructed images obtained by multi-slicing a frozen sample with the thickness of 5 μm. The values of characteristic parameters were determined for the sample images with the dimension of 530×700×1000 μm. The 3-D morphology of ice crystals was found to be a bundle of continuous or dendrite columns at any freezing condition. The equivalent diameter of ice crystals were in the range of 73–169 μm, and decreased exponentially with increasing freezing rate at the copper cooling plate temperature of −20 to −80 °C. At the Tcp−40 °C, the volumes of ice crystals were in the range of 4.6×104μm3to 3.3×107μm3, and 36 ice columns were counted in the 3-D image.