Enhanced Expression of QTL qLL9/DEP1 Facilitates the Improvement of Leaf Morphology and Grain Yield in Rice
Enhanced Expression of QTL qLL9/DEP1 Facilitates the Improvement of Leaf Morphology and Grain Yield in Rice
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QTL qLL9/DEP1增强表达有利于水稻叶片形态和籽粒产量的提高
DOI：
10.3390/ijms20040866
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发表时间：
2019-02-02
影响因子：
5.6
通讯作者：
Zhang, Guangheng
中科院分区：
文献类型：
--
作者：
Fu, Xue;Xu, Jing;Zhang, Guangheng
在超级水稻的分子育种中，必须使用大量的种质收集和遗传种群分离最佳的定量性状基因座（QTL）和叶片形状的基因并探索产量潜力。在这项研究中，使用了重组近交系（RIL）种群，该人口源自以下亲本线之间的交叉：混合米饭Chunyou84，即Japonica维护者线Chunjiang16b（CJ16）；和Indica Restorer Line Chunhui 84（C84）具有显着的叶片形态差异。在海南（HN）和Hangzhou（HZ）的不同环境条件下，在标题阶段分析了叶形性状的QTL映射。检测到叶长度的主要QTL QLL9，并使用源自单个残留杂合子（RH）的种群研究了其功能，该种群在原始种群中被鉴定出来。将QLL9划分为16.17 kb区域，该区域的两侧是分子标记C-1640和C-1642，其中包含三个开放式读取框（ORF）。我们发现，使用定量的实时聚合酶链反应（QRT-PCR），序列比较和群集的定期散布的短底滴定重复相关CAS9核酸酶（CRISPR/CAS9）基因组编辑技术，使用定量的实时聚合酶链反应（QRT-PCR），序列比较和群集定期插入的候选基因是对DEP1的。 To identify the effect of qLL9 on yield, leaf shape and grain traits were measured in near isogenic lines (NILs) NIL-qLL9(CJ16) and NIL-qLL9(C84), as well as a chromosome segment substitution line (CSSL) CSSL-qLL9(KASA) with a Kasalath introgressed segment covering qLL9 in the Wuyunjing (WYJ) 7个背景。我们的结果表明，NIL-QLL9（C84）和CSSL-QLL9（KASA）的标志叶长度分别与NIL-QLL9（CJ16）和WYJ 7的标志长度显着不同。与NIL-QLL9（CJ16）相比，NIL-QLL9（C84）的尖峰长度，晶粒尺寸和千粒重量显着更高，导致产量显着增加15.08％。类似于QLL9（C84）进行超级水稻繁殖的探索和金字塔有益基因可能会增加源（例如叶片长度和叶子面积）和水槽（例如，产量特征）。这项研究为未来研究源水稻的平衡和高收益潜力的分子机制提供了基础，从而使高产分子设计育种受益于全球粮食安全。
In molecular breeding of super rice, it is essential to isolate the best quantitative trait loci (QTLs) and genes of leaf shape and explore yield potential using large germplasm collections and genetic populations. In this study, a recombinant inbred line (RIL) population was used, which was derived from a cross between the following parental lines: hybrid rice Chunyou84, that is, japonica maintainer line Chunjiang16B (CJ16); and indica restorer line Chunhui 84 (C84) with remarkable leaf morphological differences. QTLs mapping of leaf shape traits was analyzed at the heading stage under different environmental conditions in Hainan (HN) and Hangzhou (HZ). A major QTL qLL9 for leaf length was detected and its function was studied using a population derived from a single residual heterozygote (RH), which was identified in the original population. qLL9 was delimitated to a 16.17 kb region flanked by molecular markers C-1640 and C-1642, which contained three open reading frames (ORFs). We found that the candidate gene for qLL9 is allelic to DEP1 using quantitative real-time polymerase chain reaction (qRT-PCR), sequence comparison, and the clustered regularly interspaced short palindromic repeat-associated Cas9 nuclease (CRISPR/Cas9) genome editing techniques. To identify the effect of qLL9 on yield, leaf shape and grain traits were measured in near isogenic lines (NILs) NIL-qLL9CJ16 and NIL-qLL9C84, as well as a chromosome segment substitution line (CSSL) CSSL-qLL9KASA with a Kasalath introgressed segment covering qLL9 in the Wuyunjing (WYJ) 7 backgrounds. Our results showed that the flag leaf lengths of NIL-qLL9C84 and CSSL-qLL9KASA were significantly different from those of NIL-qLL9CJ16 and WYJ 7, respectively. Compared with NIL-qLL9CJ16, the spike length, grain size, and thousand-grain weight of NIL-qLL9C84 were significantly higher, resulting in a significant increase in yield of 15.08%. Exploring and pyramiding beneficial genes resembling qLL9C84 for super rice breeding could increase both the source (e.g., leaf length and leaf area) and the sink (e.g., yield traits). This study provides a foundation for future investigation of the molecular mechanisms underlying the source–sink balance and high-yield potential of rice, benefiting high-yield molecular design breeding for global food security.