Diisopropylfluorophosphate: Suppression of Ionic Conductance of the Cholinergic Receptor
Diisopropylfluorophosphate: Suppression of Ionic Conductance of the Cholinergic Receptor
复制标题
二异丙基氟磷酸盐：胆碱能受体离子电导的抑制
DOI：
10.1126/science.181.4102.853
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发表时间：
1973
期刊：
影响因子：
56.9
通讯作者：
E. Barnard
中科院分区：
文献类型：
--
作者：
K. Kuba;Edson X. Albuquerque;E. Barnard
当青蛙缝匠肌暴露于二异丙基氟磷酸盐，终板电流的振幅和半衰减时间下降，半衰减时间变得几乎电位无关和平衡电位的终板电流是负比在控制条件下。当过量的试剂通过洗涤去除，使只有磷酸化的乙酰胆碱酯酶仍然存在，终板电流的振幅恢复，而其半衰减时间显着增加。这些研究结果表明，这种有机磷酸盐显着影响受体离子电导调制器复杂的，除了其众所周知的抗胆碱酯酶活性。
When frog sartorius muscles were exposed to diisopropylfluorophosphate, the amplitude and half-decay time of the end-plate current decreased; the half-decay time became almost potential-independent and the equilibrium potential for the end-plate current was more negative than during control conditions. When the excess reagent was removed by washing so that only the phosphorylated acetylcholinesterase remained, the amplitude of the end-plate current was restored, while its half-decay time was markedly increased. These findings reveal that this organophosphate significantly affects the receptor-ionic conductance modulator complex in addition to its well-known anticholinesterase activity.