Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia
Viability and neural differentiation of mesenchymal stem cells derived from the umbilical cord following perinatal asphyxia
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DOI：
10.1038/jp.2011.174
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发表时间：
2012-09-01
影响因子：
2.9
通讯作者：
Akmal, D.
中科院分区：
文献类型：
--
作者：
Aly, H.;Mohsen, L.;Akmal, D.
目的：缺氧缺血是世界范围内新生儿神经功能障碍的主要原因。从窒息新生儿的新鲜脐带血中收集的间充质干细胞（MSCs）具有再生受损神经组织的潜力。本研究的目的是检查骨髓间充质干细胞分化成神经组织，随后可用于自体transplantation.Study设计：我们收集脐带血样本足月新生儿围产期低氧血症（n = 27），健康新生儿（n = 14）和非缺氧早产儿（n = 14）的能力。分离单核细胞，计数，然后通过流式细胞术分析以评估各种干细胞群。采用塑料贴壁法分离骨髓间充质干细胞，并进行形态学分析。细胞经历免疫表型和三系分化潜能。然后将它们培养在有利于神经分化的条件下。免疫组化法检测神经胶质细胞酸性蛋白、微管蛋白III和少突胶质细胞标记物04抗体的表达。神经分化最好表现在来自缺氧缺血足月新生儿的细胞中，其中69%具有完全神经分化，31%具有部分神经分化。结论：脐血干细胞可作为窒息新生儿自体移植的重要来源。Journal of Perinatology（2012）32，671-676; doi：10.1038/jp.2011.174; 2011年12月1日在线发表
Objective: Hypoxia-ischemia is the leading cause of neurological handicaps in newborns worldwide. Mesenchymal stem cells (MSCs) collected from fresh cord blood of asphyxiated newborns have the potential to regenerate damaged neural tissues. The aim of this study was to examine the capacity for MSCs to differentiate into neural tissue that could subsequently be used for autologous transplantation.Study Design: We collected cord blood samples from full-term newborns with perinatal hypoxemia (n = 27), healthy newborns (n = 14) and non-hypoxic premature neonates (n = 14). Mononuclear cells were separated, counted, and then analyzed by flow cytometry to assess various stem cell populations. MSCs were isolated by plastic adherence and characterized by morphology. Cells underwent immunophenotyping and trilineage differentiation potential. They were then cultured in conditions favoring neural differentiation. Neural lineage commitment was detected using immunohistochemical staining for glial fibrillary acidic protein, tubulin Ill and oligodendrocyte marker 04 antibodies.Result: Mononuclear cell count and viability did riot differ among the three groups of infants. Neural differentiation was best demonstrated in the cells derived from hypoxia-ischemia term neonates, of which 69% had complete and 31% had partial neural differentiation. Cells derived from preterm neonates had the least amount of neural differentiation, whereas partial differentiation was observed in only 12%.Conclusion: These findings support the potential utilization of umbilical cord stem cells as a source for autologous transplant in asphyxiated neonates. journal of Perinatology (2012) 32, 671-676; doi:10.1038/jp.2011.174; published online 1 December 2011