Lipidomic profiling of dairy cattle oocytes by high performance liquid chromatography-high resolution tandem mass spectrometry for developmental competence markers
Lipidomic profiling of dairy cattle oocytes by high performance liquid chromatography-high resolution tandem mass spectrometry for developmental competence markers
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DOI：
10.1016/j.theriogenology.2019.11.039
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发表时间：
2020-03-01
期刊：
影响因子：
2.8
通讯作者：
Hui, Shu-Ping
中科院分区：
文献类型：
--
作者：
Chen, Zhen;Wu, Yue;Hui, Shu-Ping
采用高效液相色谱-高分辨率串联质谱法和多元统计分析相结合的方法，对不同发育能力的奶牛卵母细胞进行了脂质组学分析。在变性卵母细胞中发现了显著的脂质组学变化。变性卵母细胞中总三酰甘油比正常卵母细胞高1.8倍；但总心磷脂比正常卵母细胞低53.5%，表明能量代谢的衰减。与正常卵母细胞相比，变性卵母细胞中的三酰基甘油由更长的不饱和酰基链组成。相反，与正常卵母细胞相比，变性卵母细胞中游离脂肪酸的酰基链更短，不饱和程度更低。此外，变性卵母细胞中总磷脂酰肌醇（14.8 +/- 7.6 pmol vs. 24.8 +/- 5.5 pmol）、总磷脂酰胆碱（20.8 +/- 8.7 pmol vs. 33.5 +/- 7.2 pmol）和总浆磷脂原乙醇胺（9.0 +/- 4.7 pmol vs. 16.8 +/- 5.2 pmol）含量显著降低，表明变性卵母细胞中脂质代谢酶功能障碍。因此，三酰甘油的增加和某些磷脂种类的减少可能是卵母细胞发育能力的潜在标志。除了为研究卵母细胞发育过程中的脂质组学变化提供了一种新的方法外，本研究中的脂质组学分析还揭示了揭示脂质代谢在牛卵母细胞发育能力中的作用的潜力。(C) 2019爱思唯尔公司版权所有。
A comparative lipidomic profiling analysis of dairy cattle oocytes with different developmental competences was performed using a combination of high performance liquid chromatography-high resolution tandem mass spectrometry and multivariate statistical analysis. Significant lipidomic changes were identified in degenerating oocytes. Total triacylglycerol in the degenerating oocytes was 1.8-fold higher than that in the normal oocytes; however, total cardiolipin was 53.5% lesser than that in the normal oocytes, which indicated attenuation of energy metabolism. Compared to those in the normal oocytes, triacylglycerols in the degenerating oocytes were composed of longer and more unsaturated acyl chains. In contrast, the acyl chains in free fatty acids present in the degenerating oocytes were shorter and with lesser degree of unsaturation compared to those in the normal oocytes. Moreover, a significant decrease in degenerating oocytes were found in total phosphatidylinositol (14.8 +/- 7.6 pmol vs. 24.8 +/- 5.5 pmol), total phosphatidylcholine (20.8 +/- 8.7 pmol vs. 33.5 +/- 7.2 pmol), and total plasmalogen ethanolamine (9.0 +/- 4.7 pmol vs. 16.8 +/- 5.2 pmol), which indicated dysfunction of lipid-metabolizing enzymes in oocytes during degeneration. Thus, increase of triacylglycerols together with the decrease of certain phospholipid species could be potential markers of oocyte developmental competence. In addition to providing a new approach to investigate the lipidomic changes in oocyte development, the lipidomic profiling in the present study has revealed insights that hold potential to unravel the role of lipid metabolism in oocyte developmental competence in cattle. (C) 2019 Elsevier lnc. All rights reserved.