Relationship Between Different Subpopulations of Circulating CD4+ T-lymphocytes and Microvascular Structural Alterations in Humans
Relationship Between Different Subpopulations of Circulating CD4+ T-lymphocytes and Microvascular Structural Alterations in Humans
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DOI：
10.1093/ajh/hpw102
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发表时间：
2017-01-01
影响因子：
3.2
通讯作者：
Rizzoni, Damiano
中科院分区：
文献类型：
--
作者：
De Ciuceis, Carolina;Rossini, Claudia;Rizzoni, Damiano
免疫系统的不同组成部分，包括先天性和适应性免疫（T效应淋巴细胞和T调节淋巴细胞-Tregs）可能参与高血压的发展。此外，在动物模型中证明，TREG可以预防血管紧张素II诱导的高血压和血管损伤/炎症。然而，没有数据是目前在人类T淋巴细胞亚型和微血管结构alterations. METHODS之间可能存在的关系，为此目的，在本研究中，我们招募了24名血压正常的受试者和12名高血压患者进行择期手术干预。没有局部或全身炎症的迹象。所有患者在手术期间均接受了皮下脂肪活检。解剖皮下小阻力动脉并固定在钢丝肌描记器上，计算中膜与管腔比率（M/L）。此外，视网膜小动脉结构进行了评估，非侵入性扫描激光多普勒血流仪。通过视频显微镜评价甲襞、指背和前臂的毛细血管密度。术前采集外周血，用流式细胞术检测外周血T淋巴细胞亚群，结果显示，视网膜微血管结构指标（皮下小动脉M/L和视网膜小动脉壁腔比）与外周血TREG淋巴细胞呈显著负相关。皮下小动脉的M/L与循环Th 17淋巴细胞之间存在直接相关性。此外，总毛细血管密度与TREG效应记忆subpopulation. CONCLUSION我们的数据表明，一些淋巴细胞亚群可能与微血管重塑，确认以前的动物数据，并打开治疗的可能性。
BACKGROUNDDifferent components of the immune system, including innate and adaptive immunity (T-effector lymphocytes and T-regulatory lymphocytes-TREGs) may be involved in the development of hypertension. In addition, it was demonstrated in animal models that TREGs may prevent angiotensin II-induced hypertension and vascular injury/inflammation. However, no data are presently available in humans about possible relationships between T-lymphocyte subtypes and microvascular structural alterations.METHODSFor this purpose, in the present study, we enrolled 24 normotensive subjects and 12 hypertensive patients undergoing an elective surgical intervention. No sign of local or systemic inflammation was present. All patients underwent a biopsy of subcutaneous fat during surgery. Subcutaneous small resistance arteries were dissected and mounted on a wire myograph and the media to lumen ratio (M/L) was calculated. In addition, retinal arteriolar structure was evaluated noninvasively by scanning laser Doppler flowmetry. Capillary density in the nailfold, dorsum of the finger, and forearm were evaluated by videomicroscopy. A peripheral blood sample was obtained before surgery for assessment of T-lymphocyte subpopulations by flow cytometry.RESULTSSignificant negative correlations were observed between indices of microvascular structure (M/L of subcutaneous small arteries and wall to lumen ratio of retinal arterioles) and circulating TREG lymphocytes. A direct correlation was observed between M/L of subcutaneous small arteries and circulating Th17 lymphocytes. In addition, total capillary density was correlated with a TREG effector memory subpopulation.CONCLUSIONOur data suggest that some lymphocyte subpopulations may be related to microvascular remodeling, confirming previous animal data, and opening therapeutic possibilities.