Effects of dietary shrimp extract on growth performance, muscle fatty acid composition, and lipid metabolism-related genes of juvenile red tilapia
Effects of dietary shrimp extract on growth performance, muscle fatty acid composition, and lipid metabolism-related genes of juvenile red tilapia
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日粮虾提取物对红罗非鱼幼鱼生长性能、肌肉脂肪酸组成及脂质代谢相关基因的影响
DOI：
10.1111/jwas.12705
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发表时间：
2020
影响因子：
2.8
通讯作者：
Dong Zai-Jie
中科院分区：
文献类型：
--
作者：
Yu Zhen-Zhen;Wang Lan-Mei;Song Fei-Biao;Zhu Wen-Bin;Miao Ling-Hong;Fu Jian-Jun;Dong Zai-Jie
虾提取物（ShE）是一种含有丰富氨基酸的水生动物引诱剂。为了研究饲料中添加ShE对红罗非鱼幼鱼（初始体重：12.02 ± 0.02 g）生长和肌肉脂肪酸的影响，进行了为期62天的试验。鱼喂六个饮食含有分级水平的ShE（0%，0.5%，1%，2%，和3%）没有鱼粉（FM）和FM饮食在三个重复的组。在试验结束时，饲喂3%ShE饲料和FM饲料的红罗非鱼的最终体重、增重、特定生长率和摄食量显著高于饲喂其他饲料的红罗非鱼（p<0.05）。ShE饲料组鱼肌肉中总饱和脂肪酸含量低于FM饲料组（p<0.05）。ShE饮食组中的芥酸（C22：1）、二十碳五烯酸（C20：5 n-3; EPA）、二十二碳五烯酸（C22：5 n-3; DPA）和二十二碳六烯酸（C22：6 n-3; DHA）的含量也低于FM饮食组（p<0.05）。实验组红罗非鱼肝脏中thel基因的表达水平显著高于对照组（p<0.05）。添加0.5%、2%和3%的ShE饲料的红罗非鱼肝脏组织中的LDH基因mRNA表达水平显著高于其它各组（p<0.05）。此外，呼吸爆发活性在0.5%、1%和2%ShE饮食组中具有积极作用（p<0.05）。结果表明，饲料中添加ShE对红罗非鱼幼鱼的生长和脂质代谢相关基因有显著影响，建议在红罗非鱼幼鱼饲料中添加3%的ShE。
Shrimp extract (ShE) is one kind of aquatic animal attractant with abundant amino acids. In order to investigate the effects of dietary ShE supplementation on the growth and muscle fatty acid of juvenile red tilapia (initial weight: 12.02 ± 0.02 g), a 62‐day experiment was conducted. The fish were fed six diets containing graded levels of ShE (0%, 0.5%, 1%, 2%, and 3%) without fish meal (FM) and an FM diet in triplicate groups. At the end of the experiment, the final body weight, weight gain, specific growth rate, and feed intake of the red tilapia fed the 3% ShE diet and FM diet were significantly higher than those fed the other diets (p< .05). The total saturated fatty acid of fish muscle in the ShE diet groups was lower than that in the FM diet group (p< .05). The contents of erucic acid (C22:1), eicosapentaenoic acid (C20:5 n‐3; EPA), docosapentanoic acid (C22:5 n‐3; DPA), and docosahexaenoic acid (C22:6 n‐3; DHA) in the ShE diet groups were also lower than those in the FM diet group (p< .05). The expression level of thelplgene in red tilapia liver of experimental group was significantly higher than that of the control group (p< .05). The livermdhgene mRNA expression levels were higher in the red tilapia fed 0.5%, 2%, and 3% ShE diets than those of other groups (p< .05). Moreover, the respiratory burst activity had a positive effect in the 0.5%, 1%, and 2% ShE diet groups (p< .05). Our results suggest that dietary ShE has a significant effect on the growth and lipid metabolism‐related genes of fish, and 3% ShE was recommended to be supplemented in juvenile red tilapia diets.