Immunocytochemical localization of nitric oxide synthase in the mammalian retina
Immunocytochemical localization of nitric oxide synthase in the mammalian retina
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DOI：
10.1016/s0304-3940(99)00363-8
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发表时间：
1999-06-04
影响因子：
2.5
通讯作者：
Chun, MH
中科院分区：
文献类型：
--
作者：
Kim, IB;Lee, EJ;Chun, MH
使用针对大鼠、小鼠、豚鼠、兔和猫视网膜中神经元 NOS 的抗血清，通过免疫细胞化学和免疫印迹研究一氧化氮合酶 (NOS) 的定位。视网膜组织提取物的蛋白质印迹分析表明，155 kDa 的 NOS 免疫反应带存在于所有物种中。在大鼠、小鼠、豚鼠和兔子的视网膜中，两种类型的无长突细胞和一类移位的无长突细胞一致被NOS标记。与其他哺乳动物不同，猫视网膜中的一种无长突细胞和两种移位的无长突细胞表现出 NOS 免疫反应性。在大鼠和豚鼠的一些双极细胞、小鼠的一些丛状细胞、兔子的一些感光细胞和猫的相同Muller细胞中进一步发现NOS免疫反应性。 (C) 1999 Elsevier Science Ireland Ltd. 保留所有权利。
The localization of nitric oxide synthase (NOS) was investigated by immunocytochemistry and immunoblotting using an antiserum against neuronal NOS in the rat, mouse, guinea pig, rabbit and cat retinae. Western blot analysis of retinal tissue extracts showed that the NOS-immunoreactive band of 155 kDa was present in all species. In the rat, mouse, guinea pig and rabbit retinae, two types of amacrine cells and a class of displaced amacrine cells were consistently NOS-labeled. In the cat retina, unlike other mammals, one type of amacrine cells and two types of displaced amacrine cells showed NOS immunoreactivity. NOS immunoreactivity was further found in some bipolar cells of the rat and guinea pig, some interplexiform cells of the mouse, some photoreceptor cells of the rabbit and same Muller cells of the cat. (C) 1999 Elsevier Science Ireland Ltd. All rights reserved.