Synthesis of Branched Cyclodextrins with Pseudomonas Isoamylase
Synthesis of Branched Cyclodextrins with Pseudomonas Isoamylase
复制标题
假单胞菌异淀粉酶合成支链环糊精
DOI：
10.5458/jag1972.33.119
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发表时间：
1986
期刊：
影响因子：
--
通讯作者：
T. Utamura
中科院分区：
文献类型：
--
作者：
S. Hizukuri;Abe Junichi;N. Mizowaki;K. Koizumi;T. Utamura
以α-、β-和γ-环糊精（CD）和麦芽糖、麦芽三糖为原料，用假单胞菌异淀粉酶分别合成6-O-α-麦芽糖基-和6-O-α-麦芽三糖基-环糊精（CD）。与麦芽三糖的反应速率大于与麦芽糖的反应速率，并且随着CD尺寸的增加而增加。除了这些支链环糊精，支链己糖，61-，62-和63-麦芽三糖基麦芽三糖以1：4.5：1的比例通过两种麦芽三糖的缩合形成。因此，麦芽三糖作为侧链供体和受体都是有效的，但麦芽糖仅作为侧链供体有效。异淀粉酶对麦芽三糖基-CD形成的比活力大于支链淀粉酶。通过液相凝胶色谱和HPLC对支链环糊精和己糖进行了纯化，并通过化学、酶促和13 C-NMR光谱分析确定了它们的结构。
6-O-α-maltosyl- and 6-O-α-maltotriosyl-cyclodextrins (CDs) were produced from α-, β-, and γ-CDs and maltose and maltotriose with Pseudomonas isoamylase. The reaction rate was greater with maltotriose than with maltose, and with increasing size of CDs. Besides these branched cyclodextrins, branched hexaoses, 61-, 62-, and 63-maltotriosylmaltotriose in the 1:4.5:1 ratio were formed by the condensation of two maltotrioses. Thus, maltotriose was effective as both a side-chain donor and an acceptor as well, but maltose was only effective as a side-chain donor. The specific activities of isoamylase for the formation of maltotriosyl-CDs were greater than those of pullulanase. The branched CDs and the hexaoses were purified by liquid-gel chromatography and HPLC, and their structures were determined by chemical, enzymic and 13C-NMR spectroscopic analyses.