Cyclometalated iridium(iii) complex nanoparticles for mitochondria-targeted photodynamic therapy
Cyclometalated iridium(iii) complex nanoparticles for mitochondria-targeted photodynamic therapy
复制标题
用于线粒体靶向光动力治疗的环金属化铱（iii）复合纳米颗粒
DOI：
10.1039/d0nr03398g
复制
发表时间：
2020-07-14
期刊：
影响因子：
6.7
通讯作者：
Ma, Yuguo
中科院分区：
文献类型：
--
作者：
Lu, Huan;Jiang, Xinpeng;Ma, Yuguo
环化Ir（iii）配合物具有较长的三重态寿命和良好的光物理性质，是同时成像和光动力学治疗（PDT）的良好候选者。在此，我们合成了环化的Ir（iii）配合物Ir（tiq）（2）ppy，其三重激发态寿命为2.9 μ s，单线态氧产生量子产率约为100%（与四苯基卟啉相比）。通过与接枝有羧基端聚乙二醇的聚苯乙烯（PS-PEG）共沉淀制备Ir（tiq）（2）ppy纳米颗粒（Ir（tiq）（2）ppy NPs）以实现水溶性和靶向肿瘤的能力。对于MCF-7乳腺癌细胞，在白色光照射下，在相当低的光强度（5 mW cm（-2））下，Ir（tiq）（2）ppy NPs在低至1.6 μ g mL（-1）的浓度下具有比Ir（tiq）（2）ppy更高的PDT效率。此外，值得注意的是，Ir（tiq）（2）ppy纳米颗粒克服了Ir（tiq）（2）ppy在水溶液中的发射猝灭，因此可以通过共聚焦激光扫描显微镜（CLSM）跟踪治疗剂在线粒体中的分布。对辐射杀伤癌细胞的机制进行了研究，结果表明，光照射下产生的活性氧（ROS）诱导细胞凋亡，从而导致细胞死亡。
Cyclometalated Ir(iii) complexes, with a long triplet state lifetime and good photophysical properties, are good candidates for simultaneous imaging and photodynamic therapy (PDT). Herein, we synthesize a cyclometalated Ir(iii) complex, Ir(tiq)(2)ppy, whose triplet excited state lifetime is 2.9 mu s and singlet oxygen generation quantum yield is approximately 100% (compared to tetraphenylporphyrin). Ir(tiq)(2)ppy nanoparticles (Ir(tiq)(2)ppy NPs) are prepared to achieve water solubility and mitochondria-targeting ability by co-precipitating with polystyrene grafted with carboxyl-terminated poly(ethylene glycol) (PS-PEG). Ir(tiq)(2)ppy NPs have higher PDT efficiency than Ir(tiq)(2)ppy at concentrations as low as 1.6 mu g mL(-1)for MCF-7 breast cancer cells under white light irradiation at quite low light intensity (5 mW cm(-2)). Besides, it is worth noting that the emission quenching of Ir(tiq)(2)ppy in aqueous solution has been conquered by using Ir(tiq)(2)ppy NPs, thus the distribution of the therapeutic agents in mitochondria can be tracked by confocal laser scanning microscopy (CLSM). The mechanism of killing cancer cells under irradiation is investigated, and the results indicate that cell death is caused by mitochondria-mediated apoptosis, which is induced by the ROS generated under light irradiation.