DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM-CELLS - A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES
DISRUPTION OF THE PROTO-ONCOGENE INT-2 IN MOUSE EMBRYO-DERIVED STEM-CELLS - A GENERAL STRATEGY FOR TARGETING MUTATIONS TO NON-SELECTABLE GENES
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DOI：
10.1038/336348a0
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发表时间：
1988-11-24
期刊：
影响因子：
64.8
通讯作者：
CAPECCHI, MR
中科院分区：
文献类型：
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作者：
MANSOUR, SL;THOMAS, KR;CAPECCHI, MR
基因靶向——将染色体上的DNA序列与新引入的DNA序列同源重组——在小鼠胚胎干细胞中有望提供一种产生任何所需基因型小鼠的方法。我们描述了一个积极和消极的选择程序，丰富了2000倍的那些细胞，包含一个目标突变。该程序适用于分离hprt -和int-2 -突变体，但它应该适用于任何基因
Gene targeting—homologous recombination of DNA sequences residing in the chromosome with newly introduced DNA sequences—in mouse embryo-derived stem cells promises to provide a means to generate mice of any desired genotype. We describe a positive and negative selection procedure that enriches 2,000-fold for those cells that contain a targeted mutation. The procedure was applied to the isolation ofhprt−andint-2−mutants, but it should be applicable to any gene