Isolation and identification of Salmonella pullorum bacteriophage YSP2 and its use as a therapy for chicken diarrhea
Isolation and identification of Salmonella pullorum bacteriophage YSP2 and its use as a therapy for chicken diarrhea
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鸡白痢沙门氏菌噬菌体YSP2的分离鉴定及其治疗鸡腹泻的应用
DOI：
10.1007/s11262-018-1549-0
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发表时间：
2018-06-01
期刊：
影响因子：
1.6
通讯作者：
Feng, Xin
中科院分区：
文献类型：
--
作者：
Tie, Kunyuan;Yuan, Yuyu;Feng, Xin
新城疫沙门氏菌是危害发展中国家养殖业的主要病原菌，该菌引起的鸡腹泻的治疗难度越来越大。本研究分离并鉴定了一株能特异性感染沙门氏菌的强毒噬菌体YSP2。噬菌体YSP2属于菱形病毒科，潜伏期短，为10min。YSP2基因组中不存在与细菌毒力或溶酶发生相关的ORF，使其有资格用于噬菌体治疗。在鸡腹泻模型中，研究了噬菌体对鸡白僵菌引起的腹泻的治疗作用。结果表明，鸡白僵菌口服后2 h口服YSP2(1 × 1010pfu/m L，80μL/只)，以双倍半致死剂量口服，足以保护鸡的腹泻。大体观察表明，YSP2能有效减轻脏器损伤，显著减轻肠道和肝脏组织出血。此外，YSP2在稀释到108pfu/m L时仍能保持较高的疗效。鉴于YSP2对鸡腹泻的治疗效果，YSP2可作为鸡白僵菌感染的一种替代治疗策略。
Salmonella pullorum is the major pathogen that is harmful to the poultry industry in developing countries, and the treatment of chicken diarrhea caused by S. pullorum has become increasingly difficult. In this study, a virulent bacteriophage YSP2, which was able to specifically infect Salmonella, was isolated and characterized. Phage YSP2 was classified in the Siphoviridae family and had a short latent period of 10 min. No bacterial virulence- or lysogenesis-related ORF is present in the YSP2 genome, making it eligible for use in phage therapy. Experiments in vivo investigated the potential use of phages as a therapy against diarrhea in chickens caused by S. pullorum in a chicken diarrhea model, demonstrating that a single oral administration of YSP2 (1 × 1010 PFU/mL, 80 μL/chicken) 2 h after S. pullorum oral administration at a double median lethal dose was sufficient to protect chickens against diarrhea. Gross inspection showed that YSP2 can effectively reduce organ damage and significantly relieve hemorrhage in the intestine and liver tissue. Moreover, YSP2 can maintain a high curative effect when diluted to 108 PFU/mL. In light of its therapeutic effect on chicken diarrhea, YSP2 may serve as an alternative treatment strategy for infections caused by S. pullorum.