Color Representation Is Retinotopically Biased but Locally Intermingled in Mouse V1.
Color Representation Is Retinotopically Biased but Locally Intermingled in Mouse V1.
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DOI：
10.3389/fncir.2017.00022
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发表时间：
2017
影响因子：
3.5
通讯作者：
Ohki K
中科院分区：
文献类型：
--
作者：
Aihara S;Yoshida T;Hashimoto T;Ohki K
双色视觉在许多哺乳动物中很常见。然而，与三色灵长类动物相比，二色视哺乳动物初级视觉皮层（V1）的颜色处理相对未知。在这项研究中，我们调查了颜色处理的功能组织在小鼠V1。小鼠视网膜沿着其背腹轴具有两种视蛋白的分级表达模式。然而，目前尚不清楚这种表达模式是否以及如何在局部（几百微米）和区域（V1）水平上反映在皮层代表中。使用在体双光子钙（Ca 2+）成像和宽场Ca 2+成像，我们发现，V1神经元响应于S（UV）-和M（绿色）-视蛋白隔离刺激略有偏见的颜色偏好依赖于视网膜定位位置在V1。这与视网膜视蛋白的分布一致。在细胞水平上，对S-和M-视蛋白隔离刺激的偏好在数百微米的局部区域中混合在一起。这些结果表明，颜色信息的功能组织是局部混合的，但略有偏见，这取决于在小鼠V1的视网膜位置。
Dichromatic vision is common in many mammals. However, color processing in the primary visual cortex (V1) of dichromatic mammals is relatively unknown compared to the trichromatic primates. In this study, we investigated the functional organization of color processing in mouse V1. The mouse retina has a graded expression pattern of two opsins along its dorsoventral axis. However, it is not clear whether and how this expression pattern is reflected in the cortical representation at local (several hundred microns) and areal (V1) level. Using in vivo two-photon calcium (Ca2+) imaging and wide-field Ca2+ imaging, we revealed that V1 neurons responded to S (UV)- and M (green)-opsin isolating stimuli with slightly biased color preference depending on retinotopic position in V1. This was consistent with the distribution of retinal opsins. At the cellular level, preferences for S- and M-opsin isolating stimuli were intermingled in a local region encompassing several hundred microns. These results suggest that functional organizations of color information are locally intermingled, but slightly biased depending on the retinotopic position in mouse V1.