Safety and efficacy of sperm washing in HIV-1-serodiscordant couples where the male is infected: results from the European CREAThE network
Safety and efficacy of sperm washing in HIV-1-serodiscordant couples where the male is infected: results from the European CREAThE network
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DOI：
10.1097/qad.0b013e3282703879
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发表时间：
2007-09-12
期刊：
影响因子：
3.8
通讯作者：
Semprini, Augusto E.
中科院分区：
文献类型：
--
作者：
Bujan, Louis;Hollander, Lital;Semprini, Augusto E.
目的：检查对于希望在男性伴侣感染的情况下生育的 HIV-1 血清不一致夫妇进行精子清洗辅助生殖的安全性和有效性。设计和方法：一项在欧洲网络 CREAThE 的 8 个中心进行的回顾性多中心研究，涉及 1036 名希望生育的血清不一致夫妇。通过精子洗涤获得活动精子，进行了3390次辅助生殖周期（2840次宫内授精、107次体外受精、394次胞浆内单精子注射和49次冷冻胚胎移植）。在尝试辅助生殖后至少 6 个月对女性伴侣进行 HIV 检测。记录的结果指标包括辅助生殖周期数、妊娠结局和女性治疗后的艾滋病毒检测。结果：3315 个周期中总共获得了 580 例妊娠。 47 例妊娠结局未知。 533 例妊娠导致 410 例分娩和 463 例活产。 1036 名女性中，有 967 名女性在辅助生殖后进行了艾滋病毒检测（7.1% 失访）。所有记录的测试结果均为阴性。计算出的污染概率为零（95% 置信区间，0-0.09%）。 结论：这项关于精子清洗后辅助生殖的首个多中心回顾性研究表明，该方法是有效的，可以显着降低未感染女性伴侣的 HIV-1 传播风险。这些结果支持这样一种观点，即发达国家血清不一致的夫妇不能拒绝采用洗精辅助生殖技术，并且在可能的情况下，也许可以将其纳入发展中国家针对艾滋病毒的全球公共卫生倡议中。 (C) 2007 年利平科特威廉姆斯和威尔金斯。
Objective: To examine the safety and effectiveness of assisted reproduction using sperm washing for HIV-1-serodiscordant couples wishing to procreate where the male partner is infected.Design and methods: A retrospective multicentre study at eight centres adhering on the European network CREAThE and involving 1036 serodiscordant couples wishing to procreate. Sperm washing was used to obtain motile spermatozoa for 3390 assisted reproduction cycles (2840 intrauterine inseminations, 107 in-vitro fertilizations, 394 intra-cytoplasmic sperm injections and 49 frozen embryo transfers). An HIV test was performed in female partners at least 6 months after assisted reproduction attempt. The outcome measures recorded were number of assisted reproduction cycles, pregnancy outcome and HIV test on women post-treatment.Results: A total of 580 pregnancies were obtained from 3315 cycles. Pregnancy outcome was unknown in 47 cases. The 533 pregnancies resulted in 410 deliveries and 463 live births. The result of female HIV testing after assisted reproduction was known in 967 out of 1036 woman (7.1% lost to follow-up). All tests recorded were negative. The calculated probability of contamination was equal to zero (95% confidence interval, 0-0.09%).Conclusion: This first multicentre retrospective study of assisted reproduction following sperm washing demonstrates the method to be effective and to significantly reduce HIV-1 transmission risk to the uninfected female partner. These results support the view that assisted reproduction with sperm washing could not be denied to serodiscordant couples in developed countries and, where possible, could perhaps be integrated into a global public health initiative against HIV in developing countries. (C) 2007 Lippincott Williams & Wilkins.