Myocardial calcium and magnesium in acute ischemic injury.
Myocardial calcium and magnesium in acute ischemic injury.
复制标题
急性缺血性损伤中的心肌钙和镁。
DOI：
--
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发表时间：
1972
影响因子：
6
通讯作者：
R. Jennings
中科院分区：
文献类型：
--
作者：
A. C. Shen;R. Jennings
研究了缺血性损伤对犬心肌细胞中钙和镁分布的影响，实验采用的是左冠状动脉回旋支闭塞60分钟，或者闭塞40分钟后再用动脉血对受损组织进行20分钟再灌注所造成的组织损伤模型。在永久性缺血、不可逆损伤的心肌细胞中，未发现这些阳离子浓度有显著变化，但在短暂缺血发作导致死亡的细胞中，组织钙显著增加。在短暂缺血模型中，组织水和钠也增加，镁显著减少。通过细胞分级分离和化学分析以及电子显微镜和微量灰化法对增加的钙的定位进行研究，结果表明，大部分钙位于线粒体内的致密体中。在线粒体内的致密体中，钙似乎是一种尚未明确的磷酸钙形式的沉淀物。
The effect of ischemic injury on calcium and magnesium distribution in dog myocardial cells was investigated in tissue damaged by occlusion of the circumflex branch of the left coronary artery for 60 minutes or for 40 minutes followed by 20 minutes of reperfusion of the damaged tissue by arterial blood. No significant change in the concentration of these cations was noted in permanently ischemic, irreversibly injured myocardial cells, but tissue calcium was markedly increased in cells killed by an episode of transient ischemia. Tissue water and sodium also were increased and magnesium was decreased significantly in the transient ischemia model. Investigation of the localization of the increased Ca(--) by cellular fractionation and chemical analysis as well as by electron miscroscopy and microincineration showed that much of it was localized in dense bodies within the mitochondria. Within the intramitochondrial dense bodies, the calcium appeared to be a precipitate of an as yet undefined form of calcium phosphate.