Electrical stress and acid orange 7 synergistically clear the blockage of electron flow in the methanogenesis of low-strength wastewater
Electrical stress and acid orange 7 synergistically clear the blockage of electron flow in the methanogenesis of low-strength wastewater
复制标题
电场应激和酸性橙7协同清除低浓度废水产甲烷中的电子流动障碍
DOI：
10.1016/j.ese.2024.100410
复制
发表时间：
2024-03-26
影响因子：
12.6
通讯作者：
Wang，Ai-Jie
中科院分区：
文献类型：
--
作者：
Guo，Ze-Chong;Cui，Min-Hua;Wang，Ai-Jie
通过厌氧产甲烷从低浓度废水中回收能量受到有限基质可用性的限制。高效产甲烷菌群的发展至关重要，但具有挑战性。在这里，我们开发了一种策略，驯化产甲烷菌群使用导电载体（CC），电应力（ES），和酸性橙子7（AO 7）在一个修改的生物过滤器。CC、ES和AO7的协同整合促使甲烷生产速率与基线相比显著激增72倍。这种增加是由于改变产甲烷群落功能，独立的连续存在的AO7和ES。AO7作为外部电子受体，加速发酵中间产物的乙酸生成，重组细菌群落，富集电活性细菌（EAB）。与此同时，CC和ES精心策划的古菌群落的组装和促进电养产甲烷菌，通过不同的直接电化学相互作用的机制，提高嗜乙酸产甲烷的电子流。CC，ES和AO7的集体应用有效地减轻了低强度废水产甲烷中的电子流动障碍，实现了额外的34%的电子回收率。这项研究提出了一种用导电材料改进厌氧消化系统的新方法，以促进废水处理，可持续性和能源自给自足。
Energy recovery from low-strength wastewater through anaerobic methanogenesis is constrained by limited substrate availability. The development of efficient methanogenic communities is critical but challenging. Here we develop a strategy to acclimate methanogenic communities using conductive carrier (CC), electrical stress (ES), and Acid Orange 7 (AO7) in a modified biofilter. The synergistic integration of CC, ES, and AO7 precipitated a remarkable 72-fold surge in methane production rate compared to the baseline. This increase was attributed to an altered methanogenic community function, independent of the continuous presence of AO7 and ES. AO7 acted as an external electron acceptor, accelerating acetogenesis from fermentation intermediates, restructuring the bacterial community, and enriching electroactive bacteria (EAB). Meanwhile, CC and ES orchestrated the assembly of the archaeal community and promoted electrotrophic methanogens, enhancing acetotrophic methanogenesis electron flow via a mechanism distinct from direct electrochemical interactions. The collective application of CC, ES, and AO7 effectively mitigated electron flow impediments in low-strength wastewater methanogenesis, achieving an additional 34% electron recovery from the substrate. This study proposes a new method of amending anaerobic digestion systems with conductive materials to advance wastewater treatment, sustainability, and energy self-sufficiency.