PATHOGENESIS OF HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS (HEV) IN MICE EXPERIMENTALLY INFECTED BY DIFFERENT ROUTES
PATHOGENESIS OF HEMAGGLUTINATING ENCEPHALOMYELITIS VIRUS (HEV) IN MICE EXPERIMENTALLY INFECTED BY DIFFERENT ROUTES
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DOI：
10.1016/0021-9975(86)90061-7
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发表时间：
1986-11-01
影响因子：
0.8
通讯作者：
HIRANO, N
中科院分区：
文献类型：
--
作者：
YAGAMI, K;HIRAI, K;HIRANO, N
67N型血凝性脑脊髓炎病毒（HEV）脑内接种3日龄哺乳小鼠出现神经症状并死亡。该病毒在哺乳小鼠体内传代10次，命名为MB-67N株。研究了MB-67N在不同年龄小鼠和不同接种途径下的发病机制。所有接种大剂量病毒的小鼠不论年龄都死亡，尽管小剂量的病毒只在哺乳小鼠中引起致命感染。通过鼻内、腹腔和皮下接种，该病毒也能杀死16天以下的哺乳小鼠，但即使使用大剂量，也不会杀死年龄较大的小鼠。小鼠对MB-67N株的易感性受年龄和接种途径的影响。通过任何途径从患病小鼠的大脑中重新分离出高滴度的病毒，但没有从老年器官中分离出。组织学上，大脑皮层出现大量严重的局灶性坏死。免疫荧光染色和电镜观察发现，神经细胞胞质中存在特异性免疫荧光和大量病毒颗粒。这些发现表明MB-67N主要在中枢神经系统中繁殖，神经细胞是病毒复制的主要靶点。
Three-day-old suckling mice inoculated intracerebrally (i.c.) with the 67N strain of haemagglutinating encephalomyelitis virus (HEV) showed nervous signs and died. The virus was passaged 10 times in suckling mice and was designated the MB-67N strain. The pathogenesis of MB-67N was studied with various ages of mice and inoculation routes. All mice inoculated i.c. with a large dose of virus died regardless of age, although a smaller dose caused fatal infection only in suckling mice. By intranasal, intraperitoneal and subcutaneous inoculation, the virus also killed suckling mice under 16 days old, but not older mice, even with a large dose. The susceptibility of mice for the MB-67N strain was influenced by age and inoculation routes. High titres of virus were re-isolated from the brain of diseased mice after inoculation by any route, but not from older organs. Histologically, numerous areas of severe focal necrosis were produced in the cerebral cortex. Specific immuno-fluorescence and numerous viral particles were found in the cytoplasm of nerve cells by immuno-fluorescence staining and electron microscopy. These findings indicate that the MB-67N propagates mainly in the central nervous system and nerve cells serve as a main target of virus replication.