Phenotypic MicroRNA Microarrays.
Phenotypic MicroRNA Microarrays.
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DOI：
10.3390/microarrays2020063
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发表时间：
2013-04-03
期刊：
影响因子：
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通讯作者：
Soloveva V
中科院分区：
文献类型：
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作者：
Kwon YJ;Heo JY;Kim HC;Kim JY;Liuzzi M;Soloveva V
微阵列技术已经成为一种非常流行的方法，用于需要重复进行多个实验或使用各种样品进行多个实验的情况。在我们的实验室中，我们正在应用我们的高密度斑点微阵列方法，以显微镜观察瞬时引入的siRNA或cDNA对细胞形态或表型的影响。在这篇文章中，我们讨论了使用这种微尺度高通量过程来研究microRNA在选定细胞模型的生物学中的作用的可能性。在microRNA和siRNA反向转染后，microRNA产生的细胞表型与siRNA的表达无关地调节NF-κB的表达。打印microRNA分子用于反向转染到细胞中的能力为使用细胞疾病模型的microRNA文库的表型高含量筛选开辟了广阔的视野。
Microarray technology has become a very popular approach in cases where multiple experiments need to be conducted repeatedly or done with a variety of samples. In our lab, we are applying our high density spots microarray approach to microscopy visualization of the effects of transiently introduced siRNA or cDNA on cellular morphology or phenotype. In this publication, we are discussing the possibility of using this micro-scale high throughput process to study the role of microRNAs in the biology of selected cellular models. After reverse-transfection of microRNAs and siRNA, the cellular phenotype generated by microRNAs regulated NF-κB expression comparably to the siRNA. The ability to print microRNA molecules for reverse transfection into cells is opening up the wide horizon for the phenotypic high content screening of microRNA libraries using cellular disease models.