Nonclassical Monocytes Are Prone to Migrate Into Tumor in Diffuse Large B-Cell Lymphoma.
Nonclassical Monocytes Are Prone to Migrate Into Tumor in Diffuse Large B-Cell Lymphoma.
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DOI：
10.3389/fimmu.2021.755623
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发表时间：
2021
影响因子：
7.3
通讯作者：
Roussel M
中科院分区：
文献类型：
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作者：
Le Gallou S;Lhomme F;Irish JM;Mingam A;Pangault C;Monvoisin C;Ferrant J;Azzaoui I;Rossille D;Bouabdallah K;Damaj G;Cartron G;Godmer P;Le Gouill S;Casasnovas RO;Molina TJ;Houot R;Lamy T;Tarte K;Fest T;Roussel M
循环单核细胞绝对计数被认为是弥漫性大B细胞淋巴瘤(DLBCL)的独立预后因素。然而，单核细胞命名法包括具有促炎、抗炎或抑制功能的各种亚群，而它们在DLBCL中的临床相关性一直没有得到很好的探讨。在此，我们广泛评估了91例DLBCL患者的循环单核细胞异质性。CD14pos CD16neg和CD14pos CD16neg单核细胞聚集在DLBCL外周血中，呈炎症表型。外周血中非经典单核细胞(ncMOSlanpos、CD14low CD16pos Slanneg和ncMOSlanneg、CD14low CD16pos、Slanneg)减少。肿瘤条件单核细胞与DLBCL的ncMO表型相似，易于对CCL5和CXCL12的反应迁移，并与DLBCL浸润性髓系细胞相似，如质量细胞术所定义的那样。最后，我们证明了DLBCL的两个独立队列中非经典单核细胞积聚的不利影响。
Absolute count of circulating monocytes has been proposed as an independent prognostic factor in diffuse large B-cell lymphoma (DLBCL). However, monocyte nomenclature includes various subsets with pro-, anti-inflammatory, or suppressive functions, and their clinical relevance in DLBCL has been poorly explored. Herein, we broadly assessed circulating monocyte heterogeneity in 91 DLBCL patients. Classical- (cMO, CD14pos CD16neg) and intermediate- (iMO, CD14pos CD16pos) monocytes accumulated in DLBCL peripheral blood and exhibited an inflammatory phenotype. On the opposite, nonclassical monocytes (ncMOSlanpos, CD14low CD16pos Slanneg and ncMOSlanneg, CD14low CD16pos, Slanneg) were decreased in peripheral blood. Tumor-conditioned monocytes presented similarities with ncMO phenotype from DLBCL and were prone to migrate in response to CCL5 and CXCL12, and presented similarities with DLBCL-infiltrated myeloid cells, as defined by mass cytometry. Finally, we demonstrated the adverse value of an accumulation of nonclassical monocytes in 2 independent cohorts of DLBCL.