Comparative Study of Far-Field and Near-Field Raman Spectra from Silicon-Based Samples and Biological Nanostructures
Comparative Study of Far-Field and Near-Field Raman Spectra from Silicon-Based Samples and Biological Nanostructures
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DOI：
10.1021/jp206659z
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发表时间：
2011-12-22
影响因子：
3.7
通讯作者：
Hermelink, Antje
中科院分区：
文献类型：
--
作者：
Hermann, Peter;Fabian, Heinz;Hermelink, Antje
生物纳米结构的拉曼光谱表征需要近场技术，同时提供纳米级分辨率和高灵敏度。尖端增强拉曼光谱提供了从这种小结构中获得化学和结构信息所需的灵敏度。然而，当比较从样品位置获得的光谱信息时，即使相隔几纳米，近场光谱通常也会显示出显着的强度变化和带移。在本研究中，我们比较了硅基样品和生物纳米结构（如鸟痘病毒或淀粉样原纤维）的远场和近场拉曼光谱。结果表明，相对于远场光谱，尖端增强光谱的谱带宽度较窄。此外，在近场拉曼光谱中观测到的光谱变化受到样品结构和化学非均质性的强烈影响。
Raman spectroscopic characterization of biological nanostructures requires near-field techniques, which provide nanoscale resolution and high sensitivity simultaneously. Tip-enhanced Raman spectroscopy provides the required sensitivity to obtain chemical and structural information from such small structures. However, near-field spectra typically show significant intensity variations and band shifts when comparing the spectroscopic information acquired from sample positions even a few nm apart. In the present study, we compare far-field and near-field Raman spectra of silicon-based samples and biological nanostructures like avipox virus or amyloid fibrils. It is found that the width of the bands in tip-enhanced spectra is typically narrower than in the corresponding far-field spectra. Additionally, the observed spectral variations in near-field Raman spectra are strongly influenced by the structural and chemical heterogeneity of the sample.