Polycomb group proteins ESC and E(Z) are present in multiple distinct complexes that undergo dynamic changes during development
Polycomb group proteins ESC and E(Z) are present in multiple distinct complexes that undergo dynamic changes during development
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DOI：
10.1002/gene.10173
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发表时间：
2003-02-01
期刊：
影响因子：
1.5
通讯作者：
Harte, PJ
中科院分区：
文献类型：
--
作者：
Furuyama, T;Tie, F;Harte, PJ
Polycomb组蛋白是长期稳定维持转录抑制状态所必需的。已经鉴定出两个不同的Polycomb Group复合物，一个2-MDa PRC1复合物和一个600-kDa复合物，包含ESC和E(Z)蛋白以及组蛋白去乙酰化酶RPD3和组蛋白结合蛋白p55。我们在这里报告了至少有两个胚胎ESC/E(Z)复合物在发育过程中经历动态变化，第三个幼虫E(Z)复合物在ESC消失后形成。我们已经鉴定出含有RPD3和p55以及E(Z)的更大的胚胎ESC复合物，它只存在于胚胎发生中期，而先前鉴定的600-kDa的ESC/E(Z)复合物持续到胚胎发生结束。组成性过表达ESC不会促进较大或较小的胚胎复合物的异常持续，也不会延迟E(Z)与较小的ESC复合物的分离或延迟幼虫E(Z)复合物的出现，这表明这些变化是发育编程的，而不是由ESC本身的时间分布调节的。遗传去除ESC可阻止E(Z)在较小的胚胎复合体中出现，但似乎不影响较大的胚胎ESC复合体或PRC1复合体的形成。我们还发现，ESC复合物已经在前胚层胚胎中与染色体结合，并提供遗传证据表明，ESC在这个非常早期的阶段是必需的。(C) 2003 Wiley-Liss, Inc。
The Polycomb Group proteins are required for stable long-term maintenance of transcriptionally repressed states. Two distinct Polycomb Group complexes have been identified, a 2-MDa PRC1 complex and a 600-kDa complex containing the ESC and E(Z) proteins together with the histone deacetylase RPD3 and the histone-binding protein p55. We report here that there are at least two embryonic ESC/E(Z) complexes that undergo dynamic changes during development and a third larval E(Z) complex that forms after disappearance of ESC. We have identified a larger embryonic ESC complex containing RPD3 and p55, along with E(Z), that is present only until mid-embryogenesis, while the previously identified 600-kDa ESC/E(Z) complex persists until the end of embryogenesis. Constitutive overexpression of ESC does not promote abnormal persistence of the larger or smaller embryonic complexes and does not delay a dissociation of E(Z) from the smaller ESC complex or delay appearance of the larval E(Z) complex, indicating that these changes are developmentally programmed and not regulated by the temporal profile of ESC itself. Genetic removal of ESC prevents appearance of E(Z) in the smaller embryonic complex, but does not appear to affect formation of the large embryonic ESC complex or the PRC1 complex. We also show that the ESC complex is already bound to chromosomes in preblastoderm embryos and present genetic evidence that ESC is required during this very early period. (C) 2003 Wiley-Liss, Inc.