Contribution of EAG to excitability and potassium currents in Drosophila larval motoneurons.
Contribution of EAG to excitability and potassium currents in Drosophila larval motoneurons.
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DOI：
10.1152/jn.00201.2011
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发表时间：
2012-05
影响因子：
2.5
通讯作者：
S. Srinivasan;Kimberley Lance;R. Levine
中科院分区：
文献类型：
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作者：
S. Srinivasan;Kimberley Lance;R. Levine
神经元中K（+）通道表达的多样性允许广泛的兴奋性、生长和功能调节。Ether-à-go-go（EAG）是一种电压门控K（+）通道，其特征首先在于果蝇突变体中神经末梢的自发放电和增强的神经递质释放。虽然这种蛋白质具有多种功能，但其在神经元中的作用仍然知之甚少。本研究的目的是在果蝇幼虫运动神经元的EAG原位功能的特点。从胞体进行的全细胞膜片钳记录显示，在eag突变体和靶向eag RNAi表达中，I（Av）和I（Kv）K（+）电流减少。自发的尖峰样事件在eag突变体中观察到，但在野生型运动神经元中没有。因此，我们的研究结果提供的证据表明，EAG代表一个独特的K（+）通道，有助于多个K（+）电流的运动神经元，帮助调节兴奋性，与以前的观察结果在果蝇幼虫肌肉。
Diversity in the expression of K(+) channels among neurons allows a wide range of excitability, growth, and functional regulation. Ether-à-go-go (EAG), a voltage-gated K(+) channel, was first characterized in Drosophila mutants by spontaneous firing in nerve terminals and enhanced neurotransmitter release. Although diverse functions have been ascribed to this protein, its role within neurons remains poorly understood. The aim of this study was to characterize the function of EAG in situ in Drosophila larval motoneurons. Whole cell patch-clamp recordings performed from the somata revealed a decrease in I(Av) and I(Kv) K(+) currents in eag mutants and with targeted eag RNAi expression. Spontaneous spike-like events were observed in eag mutants but absent in wild-type motoneurons. Thus our results provide evidence that EAG represents a unique K(+) channel contributing to multiple K(+) currents in motoneurons helping to regulate excitability, consistent with previous observations in the Drosophila larval muscle.