Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding deltaEF1.
Negative modulation of the chicken infectious anemia virus promoter by COUP-TF1 and an E box-like element at the transcription start site binding deltaEF1.
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DOI：
10.1099/vir.0.2008/003103-0
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发表时间：
2008-12
期刊：
影响因子：
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通讯作者：
Myrna M. Miller;K. Jarosinski;K. Schat
中科院分区：
文献类型：
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作者：
Myrna M. Miller;K. Jarosinski;K. Schat
在鸡传染性贫血病毒（CAV）的启动子-增强子控制下，增强型绿色荧光蛋白（EGFP）的表达在雌激素受体增强的细胞系中用雌激素处理时增加，并且启动子-增强子结合识别共有雌激素反应元件（ERE）的未鉴定的蛋白质。CAV启动子和核受体鸡卵清蛋白上游启动子转录因子1（COUP-TF 1）的共转染实验表明，在DF-1和LMH细胞中，EGFP的表达降低了50%至60%。包括转录起始点处和下游的序列的CAV启动子比短启动子构建体具有更少的表达。在该位点假定的E盒突变恢复了表达水平。电迁移率变动分析表明，转录调节因子delta-EF 1（deltaEF 1）结合到这个E盒区域。这些发现表明CAV启动子活性可以直接或间接地受到COUP-TF 1和deltaEF 1的影响。
Expression of enhanced green fluorescent protein (EGFP) under control of the promoter-enhancer of chicken infectious anemia virus (CAV) is increased in an oestrogen receptor-enhanced cell line when treated with oestrogen and the promoter-enhancer binds unidentified proteins that recognize a consensus oestrogen response element (ERE). Co-transfection assays with the CAV promoter and the nuclear receptor chicken ovalbumin upstream promoter transcription factor 1 (COUP-TF1) showed that expression of EGFP was decreased by 50 to 60 % in DF-1 and LMH cells. The CAV promoter that included sequences at and downstream of the transcription start point had less expression than a short promoter construct. Mutation of a putative E box at this site restored expression levels. Electromobility shift assays showed that the transcription regulator delta-EF1 (deltaEF1) binds to this E box region. These findings indicate that the CAV promoter activity can be affected directly or indirectly by COUP-TF1 and deltaEF1.