H 2 SO 4 and SO 3 Transfer Reactions in a Sulfopeptide-Basic Peptide Complex
H 2 SO 4 and SO 3 Transfer Reactions in a Sulfopeptide-Basic Peptide Complex
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硫肽-碱性肽复合物中的 H 2 SO 4 和 SO 3 转移反应
DOI：
10.1021/acs.analchem.5b02479
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发表时间：
2015
影响因子：
7.4
通讯作者：
Polfer, Nicolas C.
中科院分区：
文献类型：
--
作者：
Patrick, Amanda L.;Polfer, Nicolas C.
我们报道了硫酸（H2SO4）和三氧化硫（SO3）从酸性硫肽（sSE）到碱性肽（R3）的分子间转移；这是通过使sSE + r3的非共价配合物在四极离子阱中碰撞激活来实现的。通过ms3实验对亚砜基转移产物离子进行了表征。另外，同位素标记和能量分辨碰撞诱发的离解（CID）实验也支持峰分配。讨论了观察到的反应及其对蛋白质组学和翻译后修饰发现实验的潜在意义。
We report on the intermolecular transfer of sulfuric acid (H2SO4) and sulfur trioxide (SO3) from an acidic sulfopeptide (sSE) to a basic peptide (R3); this is achieved by subjecting a noncovalent complex of sSE + R3to collisional activation in a quadrupole ion trap. The product ions resulting from the sulfo-group transfers were characterized by MS3experiments. Peak assignments were additionally supported by isotope-labeling and energy-resolved collision induced dissiciation (CID) experiments. The observed reactions and their potential implications for proteomics and post-translational modification discovery experiments are discussed.