Decreased in vitro humoral immune responses in aged humans.
Decreased in vitro humoral immune responses in aged humans.
复制标题
老年人体外体液免疫反应降低。
DOI：
10.1172/jci110122
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Good,RA
中科院分区：
文献类型：
--
作者：
Pahwa,SG;Pahwa,RN;Good,RA
在老年（65-85岁）和年轻（20-30岁）志愿者的外周血单核细胞中研究了体外抗原特异性和非特异性（多克隆）体液免疫应答的诱导。在最近描述的微量培养系统中进行淋巴细胞与抗原（绵羊红细胞）的体外免疫，并在直接溶血空斑试验中定量抗绵羊红细胞空斑形成细胞。免疫球蛋白分泌细胞，诱导多克隆与美洲商陆有丝分裂原，定量在反向溶血空斑试验。抗原特异性以及多克隆反应的显着抑郁症，注意到与年龄的增长。抗原特异性反应比多克隆反应更频繁地被抑制。T细胞有丝分裂原伴刀豆球蛋白A（Con A）用于扩增自体免疫调节T细胞的功能。在培养开始时向年轻供体的淋巴细胞中加入10 μ g/ml Con A导致抑制细胞的激活和抗原特异性应答的消除。另一方面，延迟添加Con A增强了反应，可能是因为辅助性T细胞的激活。类似的操作淋巴细胞培养物从老年供体显示失败的Con A抑制抗原特异性反应在大约一半的应答者。在许多无应答者中，正常范围内的应答是由延迟添加Con A到淋巴细胞培养物中引起的。在美洲商陆有丝分裂原刺激的年轻细胞和老年细胞之间的共培养物中，有32.5%的细胞出现超出预期反应范围的偏差。我们的研究结果表明，年龄相关的B细胞功能缺陷往往与免疫调节性T细胞功能障碍有关，只是偶尔由于B细胞的内在缺陷。
Induction of antigen-specific and non-specific (polyclonal) humoral immune responses in vitro was investigated in peripheral blood mononuclear cells of aged (65-85 yr) and young (20-30 yr) volunteers. In vitro immunization of lymphocytes with antigen (sheep erythrocytes) was performed in a recently described microculture system, and anti-sheep erythrocyte plaque forming cells were quantitated in a direct hemolytic plaque assay. Immunoglobulin secreting cells, induced polyclonally with pokeweed mitogen, were quantitated in a reverse hemolytic plaque assay. Significant depressions of antigen-specific as well as polyclonal responses were noted in relation to advancing age. Antigen-specific responses were more frequently depressed than polyclonal responses. T cell mitogen concanavalin A (Con A) was used to amplify functions of autologous immunoregulatory T cells. Addition of 10 microgram/ml Con A to lymphocytes of young donors at culture initiation resulted in activation of suppressor cells and abrogated antigen-specific responses. Delayed addition of Con A, on the other hand, enhanced responses, presumably because of activation of helper T cells. Similar manipulations of lymphocyte cultures from aged donors showed failure of Con A to suppress antigen-specific responses in approximately half of the responders. In many nonresponders, responses within normal range were elicited by the delayed addition of Con A to their lymphocyte cultures. Deviations beyond the range of expected responses were noted in 32.5% of the co-cultures between pokeweed mitogen stimulated young and aged cells. Our findings suggest that age-related deficiencies of B cell function are frequently associated with dysfunction of immunoregulatory T cells and are only occasionally due to intrinsic defects of B cells.