Cu and Cd effects on the earthworm Lumbricus rubellus in the laboratory:: Multivariate statistical analysis of relationships between exposure, biomarkers, and ecologically relevant parameters
Cu and Cd effects on the earthworm Lumbricus rubellus in the laboratory:: Multivariate statistical analysis of relationships between exposure, biomarkers, and ecologically relevant parameters
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DOI：
10.1021/es049174x
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发表时间：
2005-03-15
影响因子：
11.4
通讯作者：
Morgan, AJ
中科院分区：
文献类型：
--
作者：
Burgos, MG;Winters, C;Morgan, AJ
这项研究旨在研究一套新的分子生物标志物作为环境状态和损害的早期预警指标的潜力。四个基因，金属硫蛋白1和2，胺氧化酶，和溶酶体相关的糖蛋白的转录反应，测定在红蚯蚓暴露于浓度增加的镉和铜在经合组织土壤。这些反应进行了比较，金属体浓度和生命周期参数：生存，蚕茧生产和增长。在浓度为人工土壤LC 50的1/3至1/10时观察到不良生理效应。多元统计，主成分分析（PCA），被用来调查不同变量之间的相关性。三个关键组成部分解释了77.6%的方差，其中组成部分1贡献了32.4%，组成部分2贡献了26.7%，组成部分3贡献了18.5%。这些组成部分分别从人群健康、污染物暴露和解毒途径方面进行解释。建议使用这样一套生物标志物可以作为土壤环境“健康”的指标，并提供对生物群潜在危险的预警信号，或作为监测土壤修复的一种手段。
This study sets out to examine the potential of a suite of novel molecular biomarkers as early warning indicators of environmental state and damage. Transcriptional responses of four genes, metallothionein 1 and 2, amine oxidase, and the lysosomal associated glycoprotein, were measured in the earthworm Lumbricus rubellus exposed to increasing concentrations of cadmium and copper in OECD soil. These responses were compared to metal body concentrations and lifecycle parameters: survival, cocoon production, and growth. Adverse physiological effects were observed at concentrations 1/3rd to 1/10th those of the artificial soil LC50. Multivariate statistics, principal component analysis (PCA), was used to investigate the correlations between the different variables. Three key components were derived explaining 77.6% of the variance, with component 1 contributing 32.4%, component 2 contributing 26.7%, and component 3 contributing 18.5%. These components were interpreted in terms of population health, pollutant exposure, and detoxification pathways, respectively. It is proposed that the use of such a suite of biomarkers could serve as indicators of the "health" of the soil environment and provide early warning signals of potential danger to the biota or as a means of monitoring soil remediation.