Panel of prototypical raltegravir-resistant infectious molecular clones in a novel integrase-deleted cloning vector.
Panel of prototypical raltegravir-resistant infectious molecular clones in a novel integrase-deleted cloning vector.
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新型整合酶缺失克隆载体中的原型拉替拉韦抗性感染性分子克隆组。
DOI：
10.1128/aac.01345-09
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发表时间：
2010
影响因子：
4.9
通讯作者：
Shafer,RobertW
中科院分区：
文献类型：
--
作者：
Reuman,ElizabethC;Bachmann,MichaelH;Varghese,Vici;Fessel,WJeffrey;Shafer,RobertW
我们创建了HIV-1克隆载体pNL4.3ΔIN，以产生重组感染性分子克隆，用于分析患者来源的HIV-1整合酶编码区。使用该载体，我们构建了一组具有雷特格韦耐药突变典型模式的临床衍生病毒，并将该组提交给NIH AIDS研究和参考试剂计划。对这些克隆具有活性的研究性整合酶抑制剂可能保留对最具临床相关性的雷特格韦耐药变体的活性。
We created an HIV-1 cloning vector, pNL4.3ΔIN, to generate recombinant infectious molecular clones for analysis of patient-derived HIV-1 integrase coding regions. Using this vector, we constructed a panel of clinically derived viruses with the canonical patterns of raltegravir resistance mutations and submitted the panel to the NIH AIDS Research and Reference Reagent Program. Investigational integrase inhibitors with activity against these clones are likely to retain activity against the most clinically relevant raltegravir-resistant variants.