Disposable Microfluidic Chips for Blood Pre-Treatment and Immunoassay
Disposable Microfluidic Chips for Blood Pre-Treatment and Immunoassay
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用于血液预处理和免疫分析的一次性微流控芯片
DOI：
10.1166/sl.2011.1579
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发表时间：
2011-04
期刊：
影响因子：
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通讯作者：
X. Chen, D.F.Cui, H.Li, H.Y.Cai
中科院分区：
文献类型：
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作者：
X. Chen, D.F.Cui, H.Li, H.Y.Cai
一种带有光纤的一次性聚（二甲基硅氧烷）（PDMS）微芯片已被开发用于荧光免疫分析。采用双抗体夹心法和荧光检测法测定人IgG。在最佳免疫分析条件下，人IgG的检测范围为0.16∼20 mg/L，相关系数为0.9802。这里设计并制造了用于血液预处理和免疫分析的集成一次性 PDMS 微芯片。基于错流过滤和血浆撇渣效应，在集成微芯片中实现了等离子体隔离。这些PDMS微芯片具有成本更低、试剂和样品消耗更少、分析时间更少以及可一次性使用等优点，可实现即时临床检测的现场检测。
A disposable poly (dimethylsiloxane) (PDMS) microchip with an optical fiber has been developed for fluorescence immunoassays. Human IgG was measured by dual-antibody sandwich method and fluorescence detection. Under the optimal condition of immunoassays, the detected range of human IgG is 0.16∼20 mg/L with a correlation coefficient of 0.9802. Here an integrated disposable PDMS microchip has been designed and fabricated for the blood pre-treatment and immunoassays. Based on cross-flow filtration and plasma skimming effect, plasma isolation has been implemented in the integrated microchip. These PDMS microchips have several advantages of lower cost, lower reagent and sample consumption, less analytical time and also disposability, which enables on-site detection for point-of-care clinical testing.