Phosphorus fertilization and intercropping interactively affect tomato and potato onion growth and rhizosphere arbuscular mycorrhizal fungal community
Phosphorus fertilization and intercropping interactively affect tomato and potato onion growth and rhizosphere arbuscular mycorrhizal fungal community
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DOI：
10.1080/03650340.2020.1768530
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发表时间：
2020-06-20
影响因子：
2.4
通讯作者：
Wu, Fengzhi
中科院分区：
文献类型：
--
作者：
Gao, Danmei;Pan, Xinjie;Wu, Fengzhi
丛枝菌根真菌（AMF）在养分循环和维持农业生产力方面发挥着重要作用。虽然施磷和植物-植物相互作用都能引起AMF群落的变化，但它们的相互作用效应是否以及如何影响AMF群落尚不清楚。通过温室盆栽试验，研究了5种磷水平下马铃薯、洋葱和番茄间作系统中植物生长、磷浓度和磷含量以及丛枝菌根真菌根系定殖情况。应用定量PCR和Illumina MiSeq测序来评估AMF群落丰度和组成。与单作系统相比，间作番茄的生长和磷浓度的提高最明显，而种间相互作用对马铃薯洋葱的生长产生负面影响。间作增加了番茄AMF的丰度，但降低了马铃薯洋葱AMF的丰度，虽然高磷施肥抑制AMF的丰度，多样性和根系定殖。施磷量对两种植物AMF群落组成的影响较大，马铃薯洋葱AMF群落组成也受种植制度的影响。总体而言，番茄和马铃薯洋葱之间的种间相互作用是不对称的，对马铃薯洋葱生长的负面影响。施磷对根际AMF群落的影响强于间作，且种间互作对AMF群落的影响不对称。
Arbuscular mycorrhizal fungi (AMF) play key roles in nutrient cycling and supporting agricultural productivity. Although both phosphorus (P) fertilization and plant-plant interactions can induce changes in the AMF community, if and how their interaction effects can affect the AMF community remain unknown. A greenhouse experiment was conducted to investigate plant growth, P concentration and content and AMF root colonization in intercropped potato onion and tomato system under five P levels. Quantitative PCR and Illumina MiSeq sequencing were applied to evaluate AMF community abundance and composition. Enhanced growth and P concentration of intercropped tomato were most pronounced without P fertilization compared with monocropping system, while interspecific interactions negatively affected potato onion growth. Intercropping increased AMF abundance in tomato but decreased that in potato onion, although higher P fertilization depressed AMF abundance, diversity and root colonization. AMF community compositions of the two plants were primarily affected by P fertilization, and the AMF composition of potato onion was also separated by cropping system. Overall, interspecific interactions between tomato and potato onion were asymmetric with a negative effect on potato onion growth. P fertilization had a stronger influence than intercropping on rhizosphere AMF community, and interspecific interactions on the AMF community were not symmetric.