Myocardium at the base of the aorta and pulmonary trunk is prefigured in the outflow tract of the heart and in subdomains of the second heart field
Myocardium at the base of the aorta and pulmonary trunk is prefigured in the outflow tract of the heart and in subdomains of the second heart field
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DOI：
10.1016/j.ydbio.2007.09.023
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发表时间：
2008-01-01
影响因子：
2.7
通讯作者：
Buckingham, Margaret E.
中科院分区：
文献类型：
--
作者：
Bajolle, Fanny;Zaffran, Stephane;Buckingham, Margaret E.
小鼠心脏流出道心肌起源于前心域，即第二心域的一个子域。我们最近鉴定了一个转基因(Y96-Myf5-nlacZ-16)，它在流出道的下壁表达，然后主要在肺干底部的心肌中表达。转基因A 17-Myf5-nlacZ-T55在发育中的心脏以与Y96-Myf5-nlacZ-16互补的方式表达，在E10.5的流出道上壁和E14.5的主动脉基底部的心肌中表达。在E9.5，这两个转基因基因在心前区的不同亚区转录。对流出道心肌细胞的克隆分析，在E10.5和E14.5，提供了对心肌细胞及其前体细胞行为的洞察。在E 14.5处，大多数克隆位于肺动脉干或主动脉的底部，表明这些克隆来自不同的心肌域。在E10.5，在流出道的亚区观察到克隆。小克隆的分布表明了增殖的差异，而来自早期心肌前体细胞的大克隆的区域化反映了心脏领域以及心肌中一致的细胞生长。我们的结果表明，大动脉底部的心肌差异在前心区的不同祖细胞群中被预知，这对于了解先天性心脏病影响心脏动脉极的病因具有重要意义。(C)2007 Elsevier Inc.保留所有权利。
Outflow tract myocardium in the mouse heart is derived from the anterior heart field, a subdomain of the second heart field. We have recently characterized a transgene (y96-Myf5-nlacZ-16), which is expressed in the inferior wall of the outflow tract and then predominantly in myocardium at the base of the pulmonary trunk. Transgene A 17-Myf5-nlacZ-T55 is expressed in the developing heart in a complementary pattern to y96-Myf5-nlacZ-16, in the superior wall of the outflow tract at E10.5 and in myocardium at the base of the aorta at E14.5. At E9.5, the two transgenes are transcribed in different subdomains of the anterior heart field. A clonal analysis of cardiomyocytes in the outflow tract, at E10.5 and E14.5, provides insight into the behaviour of myocardial cells and their progenitors. At E 14.5, most clones are located at the base of either the pulmonary trunk or the aorta, indicating that these derive from distinct myocardial domains. At E10.5, clones are observed in subdomains of the outflow tract. The distribution of small clones indicates proliferative differences, whereas regionalisation of large clones, that derive from an early myocardial progenitor cell, reflect coherent cell growth in the heart field as well as in the myocardium. Our results suggest that myocardial differences at the base of the great arteries are prefigured in distinct progenitor cell populations in the anterior heart field, with important implications for understanding the etiology of congenital heart defects affecting the arterial pole of the heart. (c) 2007 Elsevier Inc. All rights reserved.