PREPROENKEPHALIN-A GENE-EXPRESSION IN RAT PINEAL
PREPROENKEPHALIN-A GENE-EXPRESSION IN RAT PINEAL
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DOI：
10.1159/000125965
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发表时间：
1991-12-01
影响因子：
4.1
通讯作者：
ALOYO, VJ
中科院分区：
文献类型：
--
作者：
ALOYO, VJ
前脑啡肽原A（ppEnk）mRNA已被确定在大鼠松果体提取物中使用寡核苷酸互补的非重叠区域的这种mRNA。 mRNA的表观分子大小为1.4 kb，相当于大鼠脑中的分子大小。 Slot-blot分析，用于确定ppEnk mRNA在几个组织中的相对量，表明松果体含有下丘脑中发现的ppEnk mRNA浓度的70%。 使用高度特异性的放射免疫测定，大鼠松果体被证明含有约3.5 pg甲硫氨酸脑啡肽（Met-Enk）每个腺体。 在酶消化以从较大分子形式释放隐藏的Met-Enk之后，松果体含有约160 pg Met-Enk/腺体。 松果体提取物的凝胶过滤分析表明，隐藏的Met-Enk峰的分子量为32 kDa（推测为脑啡肽原）。 这些结果表明，在大鼠松果体ppEnk基因产物可能有助于松果体功能。
Preproenkephalin A (ppEnk) mRNA has been identified in extracts of rat pineal gland using oligonucleotides complementary to nonoverlapping regions of this mRNA. The mRNA has an apparent molecular size of 1.4 kb, equivalent to that found in rat brain. Slot-blot analysis, used to determine the relative amount of ppEnk mRNA in serveral tissues, demonstrated that the pineal contains 70% of the concentration of ppEnk mRNA found in the hypothalamus. Using a highly specific radioimmunoassay, rat pineals were demonstrated to contain approximately 3.5 pg methionine-enkephalin (Met-Enk) per gland. Following enzymatic digestion to release cryptic Met-Enk from larger molecular forms, pineals contained approximately 160 pg Met-Enk per gland. Gel filtration analysis of pineal extracts demonstrated that the peak of the cryptic Met-Enk has a molecular weight of 32 kDa (presumably proenkephalin). These results suggest that in rat pineal ppEnk gene products may contribute to pineal functioning.