TNFAIP8 overexpression is associated with platinum resistance in epithelial ovarian cancers with optimal cytoreduction
TNFAIP8 overexpression is associated with platinum resistance in epithelial ovarian cancers with optimal cytoreduction
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DOI：
10.1016/j.humpath.2014.02.005
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发表时间：
2014-06-01
期刊：
影响因子：
3.3
通讯作者：
Lou, Ge
中科院分区：
文献类型：
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作者：
Liu, Tianbo;Xia, Bairong;Lou, Ge
在此，我们将肿瘤坏死因子α诱导蛋白8(TNFAIP8)的信使RNA(MRNA)表达与临床病理参数相关联，并探讨TNFAIP8过表达在卵巢上皮癌(EOC)铂类耐药中的作用。用Western印迹分析(n=25)和免疫组织化学方法(n=134)检测TNFAIP8蛋白的表达。用实时定量聚合酶链式反应检测新鲜冻存组织(n=40)中TNFAIP8基因的表达。铂耐药组的TNFAIP8基因和蛋白水平均明显高于铂敏感组(P<；0.05)。铂耐药与残留肿瘤大小(P=.025)、腹水(P=.027)和TNFAIP8过度表达(P=.003)独立相关。特别是，TNFAIP8的过度表达与EOCs对铂类药物的耐药性相关，细胞减少率最佳(P=.001)。TNFAIP8的表达与残存肿瘤大小密切相关(P=0.019)。总之，我们的研究结果表明，TNFAIP8的过度表达是铂耐药的独立预测因子，并可能成为靶向治疗的潜在生物标志物。(C)2014 Elsevier Inc.保留所有权利。
Here, we correlated tumor necrosis factor a induced protein 8 (TNFAIP8) messenger RNA (mRNA) expression with clinicopathological parameters and investigated the involvement of TNFAIP8 overexpression in platinum resistance of epithelial ovarian cancer (EOC). The status of TNFAIP8 protein was evaluated by Western blot analysis (n = 25) and immunohistochemistry (n = 134). TNFAIP8 mRNA expression was assessed with real-time polymerase chain reaction in fresh frozen HOC tissues (n = 40). TNFAIP8 overexpression at both mRNA and protein levels in platinum-resistant disease was clearly higher than that in platinum-sensitive disease (P < .05). Platinum resistance was independently correlated with residual tumor size (P = .025), ascites (P = .027), and TNFAIP8 overexpression (P = .003). In particular, TNFAIP8 overexpression was correlated with platinum resistance in EOCs with optimal cytoreduction (P = .001). TNFAIP8 mRNA expression was strongly associated with residual tumor size (P = .019). In conclusion, our fmdings indicate that TNFAIP8 overexpression is an independent predictor of platinum resistance and may be a potential biomarker for targeted therapy. (C) 2014 Elsevier Inc. All rights reserved.