Crucial role of Reg I from acinar-like cell cluster touching with islets (ATLANTIS) on mitogenesis of beta cells in EMC virus-induced diabetic mice
Crucial role of Reg I from acinar-like cell cluster touching with islets (ATLANTIS) on mitogenesis of beta cells in EMC virus-induced diabetic mice
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DOI：
10.1016/j.bbrc.2018.06.103
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发表时间：
2018-09-05
影响因子：
3.1
通讯作者：
Takasawa, Shin
中科院分区：
文献类型：
--
作者：
Aida, Kaoru;Kobayashi, Tetsuro;Takasawa, Shin
最近，我们报道了在人类胰腺中存在一种称为腺泡样细胞簇的细胞簇，其接触具有薄间质周围的胰岛（ATLANTIS）。一项人体形态学研究表明，ATLANTIS和胰岛细胞簇在共同的基底膜包围的微环境中一起发现，ATLANTIS释放含有再生基因蛋白的囊泡我们检测了1）纯合子Reg I α中ATLANTIS的存在或不存在，（人REG Ia的小鼠同源物）缺陷型（Reg I-/-）和野生型小鼠，2）ATLANTIS在脑心肌炎（EMC）病毒感染后β细胞簇再生中的可能作用在野生型和Reg I-/-小鼠中均发现ATLANTIS。在两组中，平均血糖在EMC病毒感染后5天短暂升高至14.0 mmol/L以上，并在12天恢复至基线水平。在EMC病毒感染后12天，与野生型小鼠相比，在Reg小鼠的胰岛β细胞中观察到较低的BrdU标记指数。EMC病毒感染Reg小鼠后12天的β细胞体积与野生型小鼠没有差异，这些结果表明，从ATLANTIS释放到胰岛β细胞簇的Reg I在EMC病毒诱导的糖尿病中的β细胞再生中具有关键作用。还表明在EMC病毒破坏后β细胞恢复中存在Reg I介导的机制以外的机制。(C)2018由Elsevier Inc.出版
Recently, we reported the presence of distinct cell clusters named acinar-like cell clusters touching Langerhans islets with thin interstitial surrounding (ATLANTIS) in human pancreas. A morphological study in humans demonstrated that ATLANTIS and islet cell clusters are found together in the micro environment enclosed by a common basement membrane, and ATLANTIS releases vesicles containing Regenerating gene protein (REG I alpha) to islet cell clusters.We examined 1) the presence or absence of ATLANTIS in homozygous Reg I (mouse homologue of human REG la) deficient (Reg I-/-) and wild-type mice, and 2) the possible role of ATLANTIS in the regeneration of beta cell clusters after encephalomyocarditis (EMC) virus (D-variant) infection in Reg and wild-type mice.ATLANTIS was found in both wild-type and Reg I-/- mice. In both groups, mean blood glucose increased transiently to greater than 14.0 mmol/L at 5 days after EMC virus infection and recovered to baseline at 12 days. At 12 days after EMC virus infection, lower BrdU labeling indices were observed in islet beta cells of Reg mice compared to wild-type mice. Beta cell volume 12 days after EMC virus infection in Reg mice did not differ from that of wild-type mice.These results suggest that Reg I, which is released from ATLANTIS to islet beta cell clusters, has a crucial role in beta cell regeneration in EMC virus-induced diabetes. The presence of mechanism(s) other than that mediated by Reg I in beta cell restoration after destruction by EMC virus was also suggested. (C) 2018 Published by Elsevier Inc.