Phytohemagglutinin-induced diarrheal disease.
Phytohemagglutinin-induced diarrheal disease.
复制标题
植物血凝素诱发的腹泻病。
DOI：
10.1007/bf01312481
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
Boldt,DH
中科院分区：
文献类型：
--
作者：
Banwell,JG;Abramowsky,CR;Weber,F;Howard,R;Boldt,DH
将纯化的植物凝集素、植物血凝素（PHA）或粗红芸豆（RKB）作为1%的膳食蛋白质添加到纯化酪蛋白饮食中，与饲喂等氮、等热量饮食的对照动物相比，断奶大鼠无法生长或体重减轻。饲喂PHA可引起腹泻：开始饲喂后2天内粪便湿重和干重均增加。粪重增加是由于干重增加和粪含水量增加引起的。在恢复正常酪蛋白饮食后，随着正常生长速率的恢复，PHA的抗营养作用迅速改善。用兔抗PHA和荧光素标记的山羊抗兔免疫球蛋白证实了PHA与小肠上皮微绒毛区特异性结合。在饮食中长期摄入或体外应用于正常组织后，观察到PHA结合。在恢复到对照酪蛋白饮食后的48小时内，观察到PHA与肠道结合的丧失。在这些实验条件下，未观察到PHA粘附对微绒毛或粘膜绒毛结构的明显形态学损害。膳食PHA的抗营养和抗吸收作用与腹泻有关。PHA在腹泻状态时粘附在小肠绒毛表面的微绒毛膜上。在饮食中停止摄入PHA后，体重增加和生长伴随着PHA粘附在小肠粘膜表面的丧失。PHA粘附在肠粘膜表面是观察到的营养和吸收不良变化的重要原因。
A purified plant lectin, phytohemagglutinin (PHA), or crude red kidney bean (RKB) from which it was derived, when incorporated as 1% of dietary protein into a purified casein protein diet caused weanling rats to fail to grow or lose weight in comparison to control animals pair fed an isonitrogenous, isocaloric diet. Feeding PHA was observed to cause diarrhea: fecal wet and dry weights were increased within 2 days after starting the diet. Increased fecal weight was caused by increased dry weight as well as by an increased fecal water content. On reversion to a normal casein diet, rapid amelioration of the antinutritional effects of PHA occurred with resumption of normal growth rate. Specific binding of PHA to the microvillus region of the small intestinal epithelium was demonstrated using rabbit anti-PHA and fluorescein-labeled goat anti-rabbit immunoglobulin. PHA binding was observed after chronic intake in the diet or when applied to normal tissuein vitro. Loss of PHA binding to the intestine was observed to occur within 48 hr on reversion to a control casein diet. No significant morphological damage to the microvilli or the mucosal villus architecture was observed to accompany PHA adherence under these experimental conditions. Antinutritional and antiabsorptive effects of dietary PHA were associated with diarrhea. PHA adhered to the microvillus membrane of the small intestinal villus surface during the diarrheal state. After cessation of dietary PHA intake, weight gain and growth was accompanied by loss of PHA adherence to the small bowel mucosal surface. Adherence of PHA to the intestinal mucosal surface was an important cause of the nutritional and malabsorptive changes observed.