Neurotransmitter-specific identification and characterization of neurons in the all-cone retina of Anolis carolinensis. II: Glutamate and aspartate.
Neurotransmitter-specific identification and characterization of neurons in the all-cone retina of Anolis carolinensis. II: Glutamate and aspartate.
复制标题
卡罗林安乐蜥全视锥视网膜神经元的神经递质特异性识别和表征。
DOI：
10.1017/s0952523800010725
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发表时间：
1992
影响因子：
1.9
通讯作者：
Ulshafer,RJ
中科院分区：
文献类型：
--
作者：
Sherry,DM;Ulshafer,RJ
使用免疫细胞化学和放射自显影方法来识别蜥蜴（Anolis carolinensis）的纯视锥细胞视网膜中可能使用谷氨酸（GLU）或天冬氨酸（ASP）作为神经递质的神经元。GLU免疫细胞化学显示所有视锥细胞类型和大量双极细胞中内源性GLU水平很高。在水平细胞和神经节细胞中发现中等 GLU 水平。穆勒细胞和大多数无长突细胞的 GLU 水平非常低。视锥细胞中的 GLU 免疫反应性 (GLU-IR) 存在于从内节到突触蒂的范围内。在近端内丛状层 (IPL) 中鉴定出具有中等 GLU-IR 的大球形细胞类型。这些细胞还含有 ASP，已初步鉴定为无长突细胞。 [3H]-L-GLU 的摄取标记了所有视网膜层。所有视锥细胞类型和 Müller 细胞都隔离 [3H]-D-ASP（一种 GLU 转运蛋白特异性底物）。在视锥细胞、水平细胞、无长突细胞和神经节细胞层细胞中观察到抗 ASP 标记。视锥细胞中的 ASP 免疫反应性 (ASP-IR) 仅限于内节。鉴定出一种在 IPL b 层中分支的含有 ASP 的梨状无长突细胞亚型。连续切片上的 GLU-IR、ASP-IR 和 GABA-IR 分析表明，Anolis 视网膜中有两种不同的水平细胞群：一种含有 GABA-IR、GLU-IR 和 ASP-IR；另一种含有 GABA-IR、GLU-IR 和 ASP-IR。另一种类型仅包含 GLU-IR 和 ASP-IR。浅色 GLU-IR 经常出现在含有 GABA 的无长突细胞中，但 ASP-IR 则不然。GLU 和 ASP 的不同分布可能表明这些氨基酸的作用截然不同。 GLU，而不是 ASP，可能是 Anolis 视网膜锥双叶神经节细胞通路中的主要神经递质。 GLU 和 ASP 均存在于水平细胞和无长突细胞的特定亚群中，但尚不清楚 GLU 或 ASP 在这些细胞中是否具有神经递质作用。
Immunocytochemical and autoradiographic methods were used to identify neurons in the pure cone retina of the lizard (Anolis carolinensis) that are likely to employ glutamate (GLU) or aspartate (ASP) as a neurotransmitter.GLU immunocytochemistry demonstrated high levels of endogenous GLU in all cone types and numerous bipolar cells. Moderate GLU levels were found in horizontal and ganglion cells. Müller cells and most amacrine cells had very low GLU levels. GLU immunoreactivity (GLU-IR) in the cones was present from the inner segment to the synaptic pedicle. A large spherical cell type with moderate GLU-IR was identified in the proximal inner plexiform layer (IPL). These cells also contain ASP and have been tentatively identified as amacrine cells. Uptake of [3H]-L-GLU labeled all retinal layers. All cone types and Müller cells sequestered [3H]-D-ASP, a substrate specific for the GLU transporter.Anti-ASP labeling was observed in cones, horizontal cells, amacrine cells, and cells in the ganglion cell layer. ASP immunoreactivity (ASP-IR) in the cones was confined to the inner segment. One ASP-containing pyriform amacrine cell subtype ramifying in IPL sublamina b was identified.Analysis of GLU-IR, ASP-IR, and GABA-IR on serial sections indicated that there were two distinct populations of horizontal cells in the Anolis retina: one containing GABA-IR, GLU-IR, and ASP-IR; and another type containing only GLU-IR and ASP-IR. Light GLU-IR was frequently found in GABA-containing amacrine cells but ASP-IR was not.The distinct distributions of GLU and ASP may indicate distinctly different roles for these amino acids. GLU, not ASP, is probably the major neurotransmitter in the cone-biploar-ganglion cell pathway of the Anolis retina. Both GLU and ASP are present in horizontal cells and specific subpopulations of amacrine cells, but it is unclear if GLU or ASP have a neurotransmitter role in these cells.