Immunomodulatory activity of polysaccharide isolated from Angelica sinensis
Immunomodulatory activity of polysaccharide isolated from Angelica sinensis
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DOI：
10.1016/j.ijbiomac.2006.02.013
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发表时间：
2006-11-15
影响因子：
8.2
通讯作者：
Mei, Qibing
中科院分区：
文献类型：
--
作者：
Yang, Tiehong;Jia, Min;Mei, Qibing
研究了从当归新鲜根中分离纯化的当归多糖（AP）的免疫调节活性。sinensis Diels）在体外研究与免疫细胞的特异性的关系。AP由鼠李糖、阿拉伯糖、甘露糖、葡萄糖、半乳糖组成，摩尔比为1.00：4.54：2.98：11.09：7.45。细胞增殖实验结果表明，AP能促进脾细胞、巨噬细胞和T细胞的增殖。AP治疗可增加IL-2和IFN-γ的产生，而减少IL-4的产生。RT-PCR分析显示，IL-2和IFN-γ基因表达增强，而IL-4基因表达降低。细胞因子的分泌模式也有一定的差异，IFN-γ的表达迅速增加，而IL-2的反应较晚。流式细胞仪检测结果显示，AP能显著提高脾细胞中CD 4 + T细胞的比例，而CD 8 + T细胞比例则略有下降。综上所述，AP通过调节Th 1和Th 2相关细胞因子的表达而发挥免疫调节作用。细胞因子反应的时间效应关系也表明，参与非特异性免疫的巨噬细胞和自然杀伤细胞主要被激活，辅助性T细胞次要受到AR（c）2006 Elsevier B. V.的影响。
The immunomodulatory activities of an Angelica sinensis polysaccharide (AP), purified from the fresh root of A. sinensis Diels, were investigated in vitro in relation to the specificity to immune cells. AP consisted of rhamnose, arabinose, mannose, glucose, galactose with the molar ratio of 1.00:4.54:2.98:11.09:7.45. Cell proliferation results showed that proliferation of total spleen cells, macrophages and T cells were promoted by the action of AP. The treatment of AP increased the production of IL-2 and IFN-gamma, while that of IL-4 was decreased. RT-PCR analysis displayed that the IL-2 and IFN-gamma gene expression were enhanced but the IL-4 gene expression was decreased. Some differences in cytokines secretion pattern were also detected, the expression of IFN-gamma was rapidly augmented while that of IL-2 responded later. The flow cytometry results showed that the percentage of CD4(+)T cell in total spleen cells was remarkably increased by AP, while that of CD8(+)T cell was slightly decreased. In conclusion, AP has immunomodulatory activity by regulating expression of Th1 and Th2 related cytokines. The time-effect relation of cytokines response also suggests that macrophages and natural killer cells involved in nonspecific immunity were primary activated, and helper T cell were secondarily affected by AR (c) 2006 Elsevier B.V. All rights reserved.