A proteomic approach to assess the host response in gills of farmed Atlantic salmon Salmo salar L. affected by amoebic gill disease
A proteomic approach to assess the host response in gills of farmed Atlantic salmon Salmo salar L. affected by amoebic gill disease
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DOI：
10.1016/j.aquaculture.2016.12.009
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发表时间：
2017-03
期刊：
影响因子：
4.5
通讯作者：
M. Marcos‐López;H. Rodger;I. O’Connor;M. Braceland;Richard J.S. Burchmore;P. Eckersall;E. MacCarthy-E.-MacCart
中科院分区：
文献类型：
--
作者：
M. Marcos‐López;H. Rodger;I. O’Connor;M. Braceland;Richard J.S. Burchmore;P. Eckersall;E. MacCarthy-E.-MacCart
阿米巴鳃病（Amoebic gill disease，AGD）是由海洋外寄生虫Neoparamoeba perurans引起的大西洋鲑（萨尔莫salar）养殖的重要传染病之一。在宿主鳃定殖后，寄生虫诱导板层上皮的显著增生和融合，如果不治疗，可能严重损害宿主。为了在蛋白质水平上研究疾病期间的宿主反应，收集连续鳃样品并使用二维电泳（2DE）进行分析，其中感兴趣的肽进行LC-MS/MS。秘鲁人。采样点设定在感染后1、2、3、7、14和21天，包括疾病的亚临床和临床阶段。LC-MS/MS共鉴定出23个蛋白质在感染和非感染个体间的差异表达，包括prohibitin、cyclophilin A、apolipoproteinA 1、ictacalcin、RhoGDP解离抑制因子α、热休克蛋白70家族成员、组蛋白H3 a和H4的表达上调，peroxiredoxin-5和cofilin的表达下调。其中最重要的蛋白质功能是细胞周期调节，细胞骨架调节，氧化代谢和免疫。这是第一个连续的蛋白质组学分析鳃在N。perurans感染。据信，使用非靶标筛选技术可以有助于了解鳃对损伤和病原性损伤（包括AGD）的反应。
Amoebic gill disease (AGD), caused by the marine ectoparasiteNeoparamoeba perurans, is one of the most significant infectious diseases for Atlantic salmon (Salmo salar) aquaculture. Upon colonisation of the host gills, the parasite induces a marked hyperplasia and fusion of the lamellar epithelium, which can severely compromise the host if left untreated. In order to investigate the host response during the disease at a protein level, sequential gill samples were collected and analysed using two-dimensional electrophoresis (2DE), with peptides of interest subjected to LC-MS/MS. Samples were obtained from an experimental challenge using naïve Atlantic salmon smolts and culturedN. perurans. Sampling points were set at 1, 2, 3, 7, 14 and 21 days post-infection, and included both sub-clinical and clinical stages of the disease. A total of 23 proteins differentially expressed between non-infected and infected individuals were successfully identified by LC-MS/MS. Findings included upregulation of prohibitin, cyclophilin A, apolipoproteinA1, ictacalcin, RhoGDP dissociation inhibitor α, components of the heat shock proteins 70 family and histones H3a and H4, and downregulation of peroxiredoxin-5 and cofilin. Among the most significant protein functions identified were cell cycle regulation, cytoskeletal regulation, oxidative metabolism and immunity. This is the first sequential proteomic analysis of gills duringN. peruransinfection. It is believed that the use of non-target screening techniques can contribute to the knowledge of gill responses to injury and pathogenic insults, including AGD.