Saliva Supernatant miR-21: a Novel Potential Biomarker for Esophageal Cancer Detection
Saliva Supernatant miR-21: a Novel Potential Biomarker for Esophageal Cancer Detection
复制标题
DOI：
10.7314/apjcp.2012.13.12.6145
复制
发表时间：
2012-01-01
影响因子：
--
通讯作者：
Li, Zi-Jun
中科院分区：
文献类型：
--
作者：
Xie, Zi-Jun;Chen, Gang;Li, Zi-Jun
目的：探讨唾液上清液miR-21能否作为食道癌新的潜在生物标志物。方法：选择32例EC患者和16例健康对照。提取唾液上清液总RNA，用RT-qPCR方法检测miR-21水平，并分析miR-21水平与EC患者临床特征的关系。结果：MIR-21在EC中的表达明显高于对照组。其敏感性和特异性分别为84.4%和62.5%。上清miR-21水平与肿瘤分期、分化程度及有无淋巴结转移无关。结论：唾液上清液miR-21可能是一种新的EC生物标志物。
Objective: To identify whether saliva supernatant miR-21 can serve as a novel potential biomarker in patients with esophageal cancer (EC). Methods: 32 patients with EC and 16 healthy controls were recruited in this study. Total RNA was extracted from saliva supernatant samples for measurement of miR-21 levels using RT-qPCR and relationships between miR-21 levels and clinical characteristics of EC patients were analyzed. Results: miR-21 was significantly higher in the EC than control groups. The sensitivity and specificity were 84.4% and 62.5% respectively. Supernatant miR-21 levels showed no significant correlation with cancer stage, differentiation and nodal metastasis. Conclusions: Saliva supernatant miR-21 may be a novel biomarker for EC.