Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays
Fluid and Highly Curved Model Membranes on Vertical Nanowire Arrays
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DOI：
10.1021/nl500926y
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发表时间：
2014-08-01
期刊：
影响因子：
10.8
通讯作者：
Nylander, Tommy
中科院分区：
文献类型：
--
作者：
Dabkowska, Aleksandra P.;Niman, Cassandra S.;Nylander, Tommy
使用垂直纳米线设备感知和操纵活细胞需要对细胞在这些底物上的行为有完整的了解。细胞功能和表型的改变通常是由质膜上发生的事件触发的，质膜的性质受到局部曲率的影响。因此，可以预期纳米线的形貌将极大地影响细胞膜，强调了研究垂直纳米线阵列上的膜的重要性。在这里，我们使用支撑型磷脂双层作为生物膜的模型。我们演示了利用溶液中的小泡自组装在垂直纳米线森林上形成流体支持的双层。发现双层膜沿着纳米线的轮廓形成连续的局部高度弯曲的模型膜。与标准的平板支撑的脂质双层不同，纳米线的高长径比导致了一个大的双层表面，可用于生物分子的固定和研究。我们使用这些双层膜将膜锚定的蛋白质和系留在纳米线底物上的囊泡结合在一起。这里展示的纳米线-双层平台可以从可控曲率衬底上的类脂膜的基础研究扩展到创新的膜基纳米传感器的开发。
Sensing and manipulating living cells using vertical nanowire devices requires a complete understanding of cell behavior on these substrates. Changes in cell function and phenotype are often triggered by events taking place at the plasma membrane, the properties of which are influenced by local curvature. The nanowire topography can therefore be expected to greatly affect the cell membrane, emphasizing the importance of studying membranes on vertical nanowire arrays. Here, we used supported phospholipid bilayers as a model for biomembranes. We demonstrate the formation of fluid supported bilayers on vertical nanowire forests using self-assembly from vesicles in solution. The bilayers were found to follow the contours of the nanowires to form continuous and locally highly curved model membranes. Distinct from standard flat supported lipid bilayers, the high aspect ratio of the nanowires results in a large bilayer surface available for the immobilization and study of biomolecules. We used these bilayers to bind a membrane-anchored protein as well as tethered vesicles on the nanowire substrate. The nanowire-bilayer platform shown here can be expanded from fundamental studies of lipid membranes on controlled curvature substrates to the development of innovative membrane-based nanosensors.