Stem cell origin of testicular seminoma.
Stem cell origin of testicular seminoma.
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DOI：
10.1016/j.clgc.2013.04.015
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发表时间：
2013-12
影响因子：
3.2
通讯作者：
Tu SM
中科院分区：
文献类型：
--
作者：
Som A;Wen S;Tu SM
关于癌症的一个主要问题是它的起源细胞。发现不同的肿瘤来源于一个层次的干细胞可能铺平道路，以证明理论的干细胞起源的癌症。据我们所知，我们第一次在人类样本中有证据表明，独特的生殖细胞肿瘤与特定的生殖系干细胞有关，这是基于它们的分子表达谱。我们假设不同的恶性亚型来自干细胞层次中的某些干细胞。关于癌症的一个主要问题是它的起源细胞。我们假设不同的癌症亚型来自独特的癌症起始细胞。通过对精原细胞瘤和精原干细胞进行微阵列荟萃分析，我们研究了精原细胞瘤的假定起源细胞。我们从基因表达综合数据库中获得了6个人类成体生殖细胞系、16个胚胎干细胞系、3个正常睾丸组织样本和40个腺瘤的已发表的微阵列数据。通过评估各种组织微阵列之间的相关性，我们确定了过渡事件的数量以及精原细胞瘤和人精原干细胞之间的距离。我们的荟萃分析显示，精原干细胞与睾丸间质细胞瘤（斯皮尔曼ρ的95% CI，0.33-0.44）和正常睾丸组织细胞（95% CI，0.39-0.40）的相关性相似，这表明细胞起源的平行途径。我们的结果分析表明，一种独特的癌症亚型，即乳腺癌，可能起源于具有干细胞特征的未分化细胞，而不是来自获得干细胞特征的分化细胞。
A major question regarding cancer is its cells of origin. The discovery of diverse tumors being derived from a hierarchy of stem cells may pave the way to prove the theory of a stem cell origin of cancers. To our knowledge, we have evidence for the first time in human samples that unique germ cell tumors are linked to particular germline stem cells on the basis of their molecular expression profile. We postulated that distinct malignant subtypes arise from certain stem cells in a stem cell hierarchy. A major question concerning cancer is its cells of origin. We hypothesized that distinct cancer subtypes arise from unique cancer-initiating cells. By performing a microarray meta-analysis of seminomas and spermatogonial stem cells, we investigated a putative cell of origin for seminoma. We obtained published microarray data for 6 human adult germ cell lines, 16 embryonic stem cell lines, 3 normal testicular tissue samples, and 40 seminomas from the Gene Expression Omnibus database. By assessing correlations between various tissue microarrays, we determined the number of transitional events and the distance between seminomas and human spermatogonial stem cells. Our meta-analysis showed that spermatogonial stem cells correlated similarly with seminoma (95% CI of Spearman ρ, 0.33–0.44) and with normal somatic testicular tissue cells (95% CI, 0.39–0.40), which suggests parallel paths of cellular origins. Analysis of our results suggests that a unique cancer subtype, namely seminoma, may have originated from an undifferentiated cell with stemness features rather than from a differentiated cell that acquired stemness features.
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DOI：
10.1098/rstb.2010.0026
发表时间：
2010-05-27
期刊：
Philosophical transactions of the Royal Society of London. Series B, Biological sciences
影响因子：
--
作者：
Phillips BT;Gassei K;Orwig KE
通讯作者：
Orwig KE
DOI：
10.1126/science.1189992
发表时间：
2010-07-30
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Goldstein AS;Huang J;Guo C;Garraway IP;Witte ON
通讯作者：
Witte ON
影响因子：
5.2
作者：
Kerr, Candace L.;Hill, Christine M.;Gearhart, John D.
通讯作者：
Gearhart, John D.
DOI：
10.1073/pnas.94.15.7719
发表时间：
1997-07-22
影响因子：
11.1
作者：
CavalliSforza, LL
通讯作者：
CavalliSforza, LL
影响因子：
4
作者：
Gashaw, Isabella;Dushaj, Oliver;Winterhager, Elke
通讯作者：
Winterhager, Elke