TATA-binding protein recognition and bending of a consensus promoter are protein species dependent.
TATA-binding protein recognition and bending of a consensus promoter are protein species dependent.
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DOI：
10.1021/bi800139w
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发表时间：
2008-06
期刊：
影响因子：
2.9
通讯作者：
Jodell E Whittington;Roberto F. Delgadillo;Torrissa J Attebury;Laura K. Parkhurst;M. Daugherty;L. Parkhurst
中科院分区：
文献类型：
--
作者：
Jodell E Whittington;Roberto F. Delgadillo;Torrissa J Attebury;Laura K. Parkhurst;M. Daugherty;L. Parkhurst
全长人TBP结合腺病毒主要晚期启动子（AdMLP）的结构和行为的特点，使用生物物理方法。人类蛋白质诱导DNA AdMLP发生97度弯曲。高分辨率的功能数据提供了一个定量的能量和动力学描述的部分反应序列作为天然人TBP迅速结合到一个共识启动子具有高亲和力。反应进行与连续形成的三个结合的物种，都具有强烈弯曲的DNA，与结合和弯曲的并发证明了荧光和各向异性停止流动。这些结果建立了TBP-DNA AdMLP结构和识别机制的蛋白质种类依赖性。此外，DNA弯曲角度和转录效率之间的强相关性，以前证明酵母TBP延伸到人TBP。异源的NH 2-末端结构域是种特异性差异的明显来源。与以前的研究一起，目前的工作建立了TBP野生型DNA TATA功能和结构依赖于TATA盒序列和TBP种类。
The structure and behavior of full-length human TBP binding the adenovirus major late promoter (AdMLP) have been characterized using biophysical methods. The human protein induces a 97 degrees bend in DNA AdMLP. The high-resolution functional data provide a quantitative energetic and kinetic description of the partial reaction sequence as native human TBP binds rapidly to a consensus promoter with high affinity. The reaction proceeds with successive formation of three bound species, all having strongly bent DNA, with the concurrence of binding and bending demonstrated by both fluorescence and anisotropy stopped flow. These results establish the protein species dependence of the TBP-DNA AdMLP structure and recognition mechanism. Additionally, the strong correlation between the DNA bend angle and transcription efficiency demonstrated previously for yeast TBP is shown to extend to human TBP. The heterologous NH 2-terminal domains are the apparent source of the species-specific differences. Together with previous studies the present work establishes that TBP wt-DNA TATA function and structure depend both on the TATA box sequence and on the TBP species.