Supporting Material
Supporting Material
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DOI：
10.4324/9780429244612-3
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发表时间：
2019-08
期刊：
影响因子：
--
通讯作者：
W. Freeden
中科院分区：
文献类型：
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作者：
W. Freeden
表S2.测定了L.酪蛋白载脂蛋白DHFR及其与甲氧苄啶（TMP）、NADPH和亚叶酸的二元和三元复合物。DHFR具有N-末端Met残基。红色表示来自配体的残基< 4 μ g的数据，黄色表示来自配体的残基<10 μ g的数据。根据干酪乳杆菌DHFR.甲氨蝶呤结构估计含亚叶酸复合物中残基的距离（33）。对无规卷曲化学位移值进行序列校正（Schwarzinger等人，2001年）。表S3. L.在20 ℃时的H和N化学位移。酪蛋白载脂蛋白DHFR及其与PABG（对氨基苯甲酰基-L-谷氨酸盐）的二元复合物：约70%的DHFR与PABG结合，与载脂蛋白DHFR达到快速交换平衡。DHFR没有N-末端Met残基。通过PABG滴定法测定标有星号的残基的apo-DHFR。
Table S2. The H and N chemical shifts at 15C of L. casei apo DHFR and its binary and ternary complexes with trimethoprim (TMP), NADPH and folinic acid. The DHFR has an N-terminal Met residue. Red colour indicates data for residues < 4 Å from ligand and yellow indicates data for residues <10 Å from ligand. Distances for residues in the folinic acid containing complexes were estimated from the L.casei DHFR.Methotrexate structure (33). The random coil chemical shift values are sequence corrected (Schwarzinger et al., 2001). Table S3.The H and N chemical shifts at 20C of L. casei apo DHFR and its binary complex with PABG (p-aminobenzoyl-L-glutamate): ~70% of the DHFR is bound to PABG in a fast-exchange equilibrium with apo DHFR. The DHFR has no N-terminal Met residue. The residues marked with an asterisk were determined for apo-DHFR by the PABG titration.