Inhibition of Carbohydrate Oxidation During the First Minute of Reperfusion After Brief Ischemia: NMR Detection of Hyperpolarized 13CO2 and H13CO3-
Inhibition of Carbohydrate Oxidation During the First Minute of Reperfusion After Brief Ischemia: NMR Detection of Hyperpolarized 13CO2 and H13CO3-
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DOI：
10.1002/mrm.21760
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发表时间：
2008-11-01
影响因子：
3.3
通讯作者：
Malloy, Craig R.
中科院分区：
文献类型：
--
作者：
Merritt, Matthew E.;Harrison, Crystal;Malloy, Craig R.
用~（31）P NMR和~（13）C NMR研究离体大鼠心脏。提供超极化[1-C]丙酮酸盐以控制含氧量正常的心脏，并以1-s时间分辨率监测[1 - 13 C]乳酸盐、[1- 13 C]丙氨酸、（CO2）-C-13和（HCO 3-）-C-13的产生。心脏还经受10分钟的全脑缺血，随后再灌注。在缺血期后3分钟内，血压、心率、耗氧量、[ATP]、[磷酸肌酸]和pH值恢复。在再灌注的前90秒，[1 -C-13]丙氨酸和[1 -C-13]乳酸迅速出现，表明丙酮酸通过主要局限于胞质溶胶的两种酶，丙氨酸氨基转移酶和乳酸脱氢酶代谢。未检出（CO2）-C-13和（HCO 3-）-C-13。缺血及再灌注后期，丙酮酸脱氢酶产物（CO_2）-C-13和（HCO_3）-C-13-易于检测。使用这种多核核磁共振方法，我们确定，在第一个90秒的再灌注PDH流量基本上是零，并在20分钟内恢复可逆性损伤的心肌。Magn Reson Med 60：1029-1036，2008. (C)2008 Wiley-Liss，Inc.
Isolated rat hearts were studied by P-31 NMR and C-13 NMR. Hyperpolarized [1-C]pyruvate was supplied to control normoxic hearts and production of [1-13C]lactate, [1-13C]alanine, (CO2)-C-13 and (HCO3-)-C-13 was monitored with 1-s temporal resolution. Hearts were also subjected to 10 min of global ischernia followed by reperfusion. Developed pressure, heart rate, oxygen consumption, [ATP], [phosphocreatine], and pH recovered within 3 min after the ischemic period. During the first 90 s of reperfusion, [1 -C-13]alanine and [1 -C-13]lactate appeared rapidly, demonstrating metabolism of pyruvate through two enzymes largely confined to the cytosol, alanine aminotransferase, and lactate dehydrogenase. (CO2)-C-13 and (HCO3-)-C-13 were not detected. Late after ischernia and reperfusion, the products of pyruvate dehydrogenase, (CO2)-C-13 and (HCO3)-C-13- were easily detected. Using this multinuclear NMR approach, we established that during the first 90 s of reperfusion PDH flux is essentially zero and recovers within 20 min in reversibly-injured myocardium. Magn Reson Med 60:1029-1036, 2008. (C) 2008 Wiley-Liss, Inc.