Ribonucleotide reductase M2 subunit expression and prognostic value in nasopharyngeal carcinoma
Ribonucleotide reductase M2 subunit expression and prognostic value in nasopharyngeal carcinoma
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核糖核苷酸还原酶M2亚基在鼻咽癌中的表达及预后价值
DOI：
10.3892/mmr.2015.3360
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发表时间：
2015-07-01
影响因子：
3.4
通讯作者：
Zeng, Mu-Sheng
中科院分区：
文献类型：
--
作者：
Han, Ping;Lin, Zhi-Rui;Zeng, Mu-Sheng
核糖核苷酸还原酶M2亚基（RRM 2）调节核糖核苷酸还原酶的酶活性，并参与肿瘤进展。最近，RRM 2高水平表达被报道与结直肠癌和膀胱癌患者的不良生存结局相关。然而，RRM 2在鼻咽癌（NPC）中的表达变化及其对该疾病预后的影响尚不清楚。本研究的目的是分析RRM 2在鼻咽癌细胞系中的表达，并确定RRM 2是否可以作为评估鼻咽癌预后的生物标志物。本研究通过逆转录-定量聚合酶链反应（RT-PCR）、免疫印迹和免疫组织化学染色发现，RRM 2在鼻咽癌细胞系和组织中的表达高于非癌鼻咽上皮细胞系和非癌组织。Kaplan-Meier生存分析表明，RRM 2表达水平较高的患者的无病生存结局比RRM 2表达水平较低的患者差。单因素分析显示，较低的生存率与RRM 2高表达水平显著相关[风险比（HR），6.424; 95%可信区间（CI），2.381-17.333; P
The ribonucleotide reductase M2 subunit (RRM2) modulates the enzymatic activity of ribonucleotide reductase, and is involved in tumor progression. Recently, high levels of RRM2 expression were reported to correlate with poor survival outcomes in patients with colorectal and bladder cancer. However, changes in RRM2 expression in nasopharyngeal carcinoma (NPC), and its effect on the prognosis of this disease remain unknown. The aim of the present study was to analyze the expression of RRM2 in NPC cell lines, and to identify whether RRM2 may serve as a biomarker with which to assess the prognosis of NPC. The present study found that RRM2 expression was higher in NPC cell lines and tissue samples than in noncancerous nasopharyngeal epithelial cell lines and noncancerous tissues, as shown by reverse transcription-quantitative polymerase chain reaction analysis, western blotting and immunohistochemistry staining. Kaplan-Meier survival analysis demonstrated that patients with higher RRM2 expression levels had poorer disease-free survival outcomes than those with lower expression levels of RRM2. Univariate analysis showed that a lower survival rate was significantly associated with high RRM2 expression levels [hazard ratio (HR), 6.424; 95% confidence interval (CI), 2.381-17.333; P