Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure
Matrix-assisted laser desorption/ionization imaging mass spectrometry revealed traces of dental problem associated with dental structure
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DOI：
10.1007/s00216-013-7075-y
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发表时间：
2014-02-01
影响因子：
4.3
通讯作者：
Setou, Mitsutoshi
中科院分区：
文献类型：
--
作者：
Hirano, Hirokazu;Masaki, Noritaka;Setou, Mitsutoshi
牙周病是一种严重的牙齿问题，因为它不会自然愈合，并导致牙齿脱落。在牙周病中，牙周组织的炎症被认为是主要的，其对牙齿本身的影响尚不清楚。在这项研究中，我们首次将基质辅助激光解吸/电离成像质谱（IMS）应用于牙齿。通过对牙周病患牙与正常牙的解剖结构进行比较，分析牙周病在牙体上的痕迹。我们发现的信号特征的釉质，牙本质和牙髓，分别从正常牙齿的质谱。使用这些信号重建的离子图像清楚地显示了牙齿的解剖结构。接下来，我们对牙周病的牙齿进行IMS。质谱的总体特征与正常质谱相似。然而，离子图像重建信号从牙周病的牙齿显示牙周膜的损失可视化与正常牙齿的牙髓。此外，离子图像清楚地描绘了根表面m/z 496.3处的信号积累。这种不能仅从质谱检查的积累通过IMS的利用被揭示。最近关于炎症的研究表明，m/z 496.3处的信号反映了溶血磷脂酰胆碱（LPC）。信号的渗透是统计学上显著的，并且其强度轮廓表现出的影响已经深入到牙齿中。这表明，牙周病的影响不仅是牙周组织的炎症，而且LPC也渗透到根表面，因此，除了常规治疗外，还需要抗炎治疗。
Periodontal disease is a serious dental problem because it does not heal naturally and leads to tooth loss. In periodontal disease, inflammation at periodontal tissue is thought as predominant, and its effect against tooth itself remains unclear. In this study, we applied matrix-assisted laser desorption/ionization imaging mass spectrometry (IMS) to teeth for the first time. By comparing anatomical structure of tooth affected with periodontal disease with normal ones, we analyzed traces of the disease on tooth. We found signals characteristic of enamel, dentin, and dental pulp, respectively, in mass spectra obtained from normal teeth. Ion images reconstructed using these signals showed anatomical structures of the tooth clearly. Next, we performed IMS upon teeth of periodontal disease. Overall characteristic of the mass spectrum appeared similar to normal ones. However, ion images reconstructed using signals from the tooth of periodontal disease revealed loss of periodontal ligament visualized together with dental pulp in normal teeth. Moreover, ion image clearly depicted an accumulation of signal at m/z 496.3 at root surface. Such an accumulation that cannot be examined only from mass spectrum was revealed by utilization of IMS. Recent studies about inflammation revealed that the signal at m/z 496.3 reflects lyso-phosphatidylcholine (LPC). Infiltration of the signal is statistically significant, and its intensity profile exhibited the influence has reached deeply into the tooth. This suggests that influence of periodontal disease is not only inflammation of periodontal tissue but also infiltration of LPC to root surface, and therefore, anti-inflammatory treatment is required besides conventional treatments.