Cloning and characterization of a human lysyl oxidase-like 3 gene (hLOXL3)
Cloning and characterization of a human lysyl oxidase-like 3 gene (hLOXL3)
复制标题
DOI：
10.1016/s0945-053x(01)00124-x
复制
发表时间：
2001-04-01
期刊：
影响因子：
6.9
通讯作者：
Mao, YM
中科院分区：
文献类型：
--
作者：
Huang, Y;Dai, JL;Mao, YM
以人表达序列标签（EST）为引物，从人胎脑mRNA中克隆了赖氨酰氧化酶基因家族新成员赖氨酰氧化酶样基因3（LOXL 3）的cDNA。预测的hLOXL 3基因的氨基酸序列与mLOR 2高度同源。生物信息学分析表明，hLOXL 3蛋白也是清道夫受体富含半胱氨酸家族的成员，含有一个25个氨基酸的信号肽。经BLAST搜索，将hLOXL 3基因定位于人2 p13位点，发现至少有14个外显子，hLOXL 3基因在人多种组织中均有表达，尤其在脾和睾丸中表达量较高。(C)2001 Elsevier Science B.V./国际矩阵生物学学会。All rights reserved.
Using the PCR primers generated from human expressed sequence tag (EST), the cDNA of lysyl oxidase-like gene 3 (LOXL3), a new member of human lysyl oxidases gene family, was cloned from the human fetal brain mRNA. The predicted amino acid sequence of the hLOXL3 gene was highly homologous to mLOR2. Bioinformatics analysis shows that hLOXL3 protein is also a member of the scavenger receptor cysteine-rich family, which contains a 25 amino acids signal peptide. The hLOXL3 gene was mapped to human 2p13 locus by BLAST search and at least 14 exons were found. Expression of the hLOXL3 gene was detected in several human tissues and especially high in spleen and testis. (C) 2001 Elsevier Science B.V./International Society of Matrix Biology. All rights reserved.